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Journal of Biological Sciences
  Year: 2013 | Volume: 13 | Issue: 4 | Page No.: 257-263
DOI: 10.3923/jbs.2013.257.263
Preparation of Chitosan Derivatives from Gladius of Squid Sepioteuthis lessoniana (Lesson, 1830) and Antimicrobial Potential against Human Pathogens
Namasivayam Subhapradha, Pasiyappazham Ramasamy, Alagiri Srinivasan, Annaian Shanmugam and Vairamani Shanmugam

Abstract:
In this study, the antimicrobial potential of chitosan and its water soluble derivatives from gladius of squid Sepioteuthis lessoniana against eleven human pathogens (gram +ve and gram -ve bacteria) was investigated. Chitosan was extracted from the gladius through demineralization, deproteinization and deacetylation. Chitosan was chemically modified by reacting with DMF-chlorosulfonic acid and orthophosphoric acid to yield water soluble derivatives such as sulfated chitosan and phosphorylated chitosan, respectively. The antibacterial activity was assessed by well diffusion technique. Different concentrations of chitosan and water soluble derivatives were analyzed by MIC techniques. The structure of chitosan, sulfated chitosan and phosphorylated chitosan was elucidated by FT-IR spectroscopy. Chitosan showed maximum inhibition of 14 mm against S. aureus and minimum inhibition of 8 mm against K. pnemoniae and V. cholerae. SCL showed maximum inhibition of 14 mm against V. cholerae and E. coli. PCL showed maximum inhibition of 13 mm against V. cholerae and S. pnemoniae. Chitosan and its derivatives possess potent antibacterial activity against several pathogens among the eleven pathogens tested and could be used as an alternative antibacterial agent.
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How to cite this article:

Namasivayam Subhapradha, Pasiyappazham Ramasamy, Alagiri Srinivasan, Annaian Shanmugam and Vairamani Shanmugam, 2013. Preparation of Chitosan Derivatives from Gladius of Squid Sepioteuthis lessoniana (Lesson, 1830) and Antimicrobial Potential against Human Pathogens. Journal of Biological Sciences, 13: 257-263.

DOI: 10.3923/jbs.2013.257.263

URL: https://scialert.net/abstract/?doi=jbs.2013.257.263

 
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