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Journal of Biological Sciences

Year: 2012 | Volume: 12 | Issue: 4 | Page No.: 258-262
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Authors

Bo Luo

Huailin Xiong

Yingyu Mao

Haiqing Yu

Keywords

Research Article

Expression of Toll-Like Receptor 2 mRNA in Spleens of Paederus Dermatitis Mice

Bo Luo, Huailin Xiong, Yingyu Mao and Haiqing Yu
Currently, it is obscure whether Toll-like Receptor 2 (TLR2) is related to the pathogenesis and pathophysiology of Paederus Dermatitis (PD). This study focused on the analysis of TLR2 mRNA expression in spleen of PD mice. Pederin was extracted at -20°C by dipping six female Paederus fuscipes Curtis in 1 mL of 70% ethanol for one month. Mouse abdominal hairs were denuded gently using 4% Na2S. By evenly extending 20 μL pederin solutions in 8 mm diameter skin, PD were induced in mice. Erythema and edema symptoms were scored. At 44 h after induction, the expression of TLR2 mRNA in spleen was analyzed using a reverse transcription and semi-quantitative polymerase chain reaction (SQ-PCR) with GAPDH mRNA as a reference. Normal mice with unhaired abdominal skin were used as controls. In this study, erythema and edema symptoms were observable, especially at 20 h after pederin application. The relative quantity of TLR2/GAPDH mRNA was 1.32±0.213 and 0.896±0.036 in healthy group and PD group, respectively. The expression level of TLR2 mRNA in spleen was significantly decreased (p<0.05) after treatment with pederin. These results suggested that pederin is a Th1 (T helper cell 1) inducing agent and there are Th1 cytokines in PD mice in early stage after contact, however, down-regulation of TLR2 mRNA expression is most likely related to Th2 (T helper cell 2) cytokines. The pathogenesis and pathophysiology In PD mice are closely associated with Th1 and Th2 responses, especially with cytokines excretion. This hypothesis needs to be investigated further.
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