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Journal of Biological Sciences

Year: 2012 | Volume: 12 | Issue: 4 | Page No.: 218-224
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Authors

Nor Asmara Tasrip

Mohd Nasir Mohd Desa

Cheah Yoke Kqueen

Nurul Zarith Mohamad Zin

Zainul Amiruddin Zakaria

Zarizal Suhaili

Chong Pei Pei

Mariana Nor Shamsudin

Keywords

This study compared the distribution of aerial mass colors, antibacterial activities and RAPD-based genomic patterns of 39 Streptomyces isolates harvested mainly from soil at the University Agricultural Park, Universiti Putra Malaysia. Streptomyces griseus ATCC 10137 strain was also included as a reference strain (n = 40). Based on ISP-2 media, the aerial mass colors observed were categorized as yellow (n = 15), grey (n = 9), brown (n = 7), white (n = 6) and others (n = 3). Antibacterial activities were assessed on Mueller Hinton Agar (MHA) and Tryptic Soy Agar (TSA) by perpendicular streak method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella sp. and Enterococcus sp. MHA demonstrated 15 isolates with broad spectrum antibacterial activities and 18 as non-broad spectrum. TSA gave lower proportions with 15 and 9 isolates respectively. Regardless of the test media used, a higher proportion of isolates with non-white color showed antibacterial activities to suggest a potential correlation. RAPD dendrogram (a composite of 3 random primers) also clustered majority of them but segregated those of white color, which showed less antibacterial activities, in a different cluster. Further validations involving more isolates are warranted to establish the findings.
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How to cite this article

Nor Asmara Tasrip, Mohd Nasir Mohd Desa, Cheah Yoke Kqueen, Nurul Zarith Mohamad Zin, Zainul Amiruddin Zakaria, Zarizal Suhaili, Chong Pei Pei and Mariana Nor Shamsudin, 2012. A Comparison Between Aerial Mass Colors, Antibacterial Activities and RAPD Fingerprints of Soil Isolates of Streptomyces. Journal of Biological Sciences, 12: 218-224.

DOI: 10.3923/jbs.2012.218.224

URL: https://scialert.net/abstract/?doi=jbs.2012.218.224

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