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Journal of Biological Sciences

Year: 2011 | Volume: 11 | Issue: 1 | Page No.: 58-63
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Authors

Srijata Mitra

Pranab Roy

Keywords

Research Article

Molecular Phylogeny of a Novel Trichloroethylene Degrading Gene of Bacillus cereus 2479

Srijata Mitra and Pranab Roy
Trichloroethylene (TCE) is one of the important volatile chlorinated organic compounds frequently used as solvents and metal degreasers in various industrial applications. It is a suspected carcinogen and potent mutagen in human. Bacteria that grow on hydrocarbons and able to degrade TCE typically initiate oxidation by incorporating oxygen from the atmosphere into organic compounds by the action of enzymes known as Oxygenases. As per information available on the biochemistry and genetic regulation of toluene dioxygenase in Pseudomonas putida Fl, making this gene (todC1) the best choice for bacterial TCE degradation. The phylogenetic relationships and divergence among Trichloroethylene (TCE) degrading gene sequences of different bacteria are studied. As the toluene dioxygenase C1 (todC1) is crucial gene for TCE degradation, todC1 extracted from NCBI GenBank database for phylogenetic analysis. Phylogenetic analysis of a new gene tce1 isolated from Bacillus cereus strain 2479 was done to investigate the evolutionary relationship among the toluene dioxygenase genes of different bacteria. Phylogenetic analysis involved the identification of homologous sequences, their multiple alignment, phylogenetic reconstruction and the graphical representation of the inferred tree was done in Phylogeny fr. A similar Phylogenetic analysis using deduced amino acids sequence (Tce1) was also carried out to confirm the gene family of tce1. On the basis of Phylogenetic data, it is revealed that tce1 gene belongs to dioxygenase family.
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