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Journal of Biological Sciences
  Year: 2011 | Volume: 11 | Issue: 4 | Page No.: 307-313
DOI: 10.3923/jbs.2011.307.313
Anti-mutagenic and Anti-genotoxic Effect of Ethanolic Extract of Neem on Dietary-aflatoxin Induced Genotoxicity in Mice
O.A. Alabi, C.P. Anokwuru, C.N. Ezekiel, O. Ajibaye, U.J. Nwadike, O. Fasasi and M. Abu

Abstract:
The discovery and exploration of compounds possessing anti-mutagenic and anti-carcinogenic properties are of great importance. This study investigated the anti-mutagenic and anti-genotoxic effect of ethanolic neem extract on dietary-aflatoxin induced genotoxicity. The assays used were: micronucleus (MN) and sperm morphology assays. Nine groups of mice labeled A-I were exposed to dietary-aflatoxin for three weeks after which they were treated with ethanolic neem extract. One week of 0.5 mL intraperitoneal injection of 25, 50 and 100 mg kg-1 b.wt. neem extract were administered in one, two and three doses daily for groups A-C, D-F and G-I, respectively. Three weeks aflatoxin feed mice and mice with no aflatoxin/neem extract exposure were used as controls 1 and 2, respectively. The three weeks dietary-aflatoxin induced a statistically significant (p<0.05) MN and sperm morphology compared to control 2. The three concentrations utilized reduced the genotoxic effects of dietary-aflatoxin on MN and abnormal sperm morphology assay in a concentration-dependent manner. Higher concentrations of 50 and 100 mg kg-1 b.wt. completely reversed the induced genotoxicity. Neem extract is identified with possible anti-mutagenic and anti-genotoxic potential in this study and should be further studied as a natural source of antimutagenic agent for humans.
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How to cite this article:

O.A. Alabi, C.P. Anokwuru, C.N. Ezekiel, O. Ajibaye, U.J. Nwadike, O. Fasasi and M. Abu, 2011. Anti-mutagenic and Anti-genotoxic Effect of Ethanolic Extract of Neem on Dietary-aflatoxin Induced Genotoxicity in Mice. Journal of Biological Sciences, 11: 307-313.

DOI: 10.3923/jbs.2011.307.313

URL: https://scialert.net/abstract/?doi=jbs.2011.307.313

 
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