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Journal of Biological Sciences

Year: 2011 | Volume: 11 | Issue: 4 | Page No.: 307-313
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Authors

O.A. Alabi

C.P. Anokwuru

C.N. Ezekiel

O. Ajibaye

U.J. Nwadike

O. Fasasi

M. Abu

Keywords

The discovery and exploration of compounds possessing anti-mutagenic and anti-carcinogenic properties are of great importance. This study investigated the anti-mutagenic and anti-genotoxic effect of ethanolic neem extract on dietary-aflatoxin induced genotoxicity. The assays used were: micronucleus (MN) and sperm morphology assays. Nine groups of mice labeled A-I were exposed to dietary-aflatoxin for three weeks after which they were treated with ethanolic neem extract. One week of 0.5 mL intraperitoneal injection of 25, 50 and 100 mg kg-1 b.wt. neem extract were administered in one, two and three doses daily for groups A-C, D-F and G-I, respectively. Three weeks aflatoxin feed mice and mice with no aflatoxin/neem extract exposure were used as controls 1 and 2, respectively. The three weeks dietary-aflatoxin induced a statistically significant (p<0.05) MN and sperm morphology compared to control 2. The three concentrations utilized reduced the genotoxic effects of dietary-aflatoxin on MN and abnormal sperm morphology assay in a concentration-dependent manner. Higher concentrations of 50 and 100 mg kg-1 b.wt. completely reversed the induced genotoxicity. Neem extract is identified with possible anti-mutagenic and anti-genotoxic potential in this study and should be further studied as a natural source of antimutagenic agent for humans.
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