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Journal of Biological Sciences

Year: 2011 | Volume: 11 | Issue: 4 | Page No.: 288-298
DOI: 10.3923/jbs.2011.288.298

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Authors


Siti A. Budhiyanti

Country: Indonesia

Sri Raharjo

Country: Indonesia

Djagal W. Marseno

Country: Indonesia

Iwan Y.B. Lelana

Country: Indonesia

Keywords


  • singlet oxygen quencher
  • metal chelator
  • free radical scavenger
  • antioxidant activity
  • Sargassum hystrix
Research Article

Free Radical Scavenging, Metal Chelating and Singlet Oxygen Quenching Activity of Fractionated Brown Seaweed Sargassum hystrix Extract

Siti A. Budhiyanti, Sri Raharjo, Djagal W. Marseno and Iwan Y.B. Lelana
The objective of the study was to isolate phenolic compound from the Sargassum hystrix crude extract by sequential solvent extraction using ethyl acetate, dichloromethane and butanol. In vitro antioxidant activity of fractionated cytoplasmic and membrane bound extracts were investigated. In addition to total phenolic content, the antioxidant activity were studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, metal chelating ability and Singlet Oxygen Quenching (SOQ) activity. Singlet oxygen quenching activity was examined using linoleic acid as substrate, containing 100 ppm erythrosine as a photosensitizer. The results showed that the membrane bound fractions had higher total phenolic compound, radical scavenging activity, ferrous ion-chelating ability and singlet oxygen quenching activity than cytoplasmic fractions (p<0.05). Among the eight fractions from membrane bound and cytoplasmic extracts isolated by differential solvent extraction, aqueous and butanol fractions of membrane bound extracts showed the highest DPPH radical scavenging, metal chelating ability and singlet oxygen quenching activity. The IC50 and metal chelating activity of aqueous fractions were 0.27±0.02 mg mL-1 and 51.53±6.63%, respectively and butanol fractions were 0.33±0.03 mg mL-1 and 44.75±3.33%, respectively. The butanol and aqueous fractions could act as SOQ at 75 ppm and 80 ppm, respectively. These results suggested that butanol and aqueous fractions were the most potent radical scavenger, metal chelator and singlet oxygen quencher.
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How to cite this article

Siti A. Budhiyanti, Sri Raharjo, Djagal W. Marseno and Iwan Y.B. Lelana, 2011. Free Radical Scavenging, Metal Chelating and Singlet Oxygen Quenching Activity of Fractionated Brown Seaweed Sargassum hystrix Extract. Journal of Biological Sciences, 11: 288-298.

DOI: 10.3923/jbs.2011.288.298

URL: https://scialert.net/abstract/?doi=jbs.2011.288.298

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