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Journal of Biological Sciences
  Year: 2011 | Volume: 11 | Issue: 3 | Page No.: 254-260
DOI: 10.3923/jbs.2011.254.260
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In vitro Rapid Propagation of Cymbidium aloifolium (L.) Sw.:A Medicinally Important Orchid via Seed Culture

Potshangbam Nongdam and Nirmala Chongtham

Cymbidium aloifolium (L.) Sw., an epiphytic orchid is one of the rare orchids of North-Eastern India having high medicinal and ornamental values. The present study was conducted in an attempt to preserve this important orchid by establishing an efficient in vitro regeneration protocol using seed culture. Two culture media viz., Mitra (M) and Murashige and Skoog (MS) supplemented with different plant growth regulators like Indole-3-butyric acid (IBA), 6-Benzyl Amino Purine (BAP) and 1-Nathyl Acetic Acid (NAA) singly or in combination were used for the present study. In 0.5 mg L-1 BAP supplemented media, seed germination percentage was very low and seedling formation was not observed. A similar response was observed when a combination of both IBA and BAP at 0.5 mg L-1 was used. Protocorm development was delayed and insignificant protocorms turned brown in color with the lost of chlorophyll and finally died even after repeated subculturing. Presence of Activated Charcoal (AC) significantly improved seed germination, protocorm formation and shoot multiplication. Shooting was best observed in MS medium supplemented with 1 mg L-1 BAP and AC. Auxins like NAA and IBA proved to be beneficial for rapid root initiation. However, more superior rooting was observed when M medium was enriched with 0.5 mg L-1 of NAA. Healthy seedlings with well developed roots were obtained which were then hardened and transplanted to community pots for further acclimatization to the nursery conditions. The regenerated plants can subsequently be used for commercial and conservation purposes.
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  •    Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds
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  •    In vitro Seed Propagation of Dendrobium (Dendrobium transparens) Orchid as Influenced by Different Media
  •    Effects of Plant Growth Regulators on Callus Proliferation, Plantlet Regeneration and Growth of Plantlets of Doritaenopsis Orchid
  •    Protocorm Regeneration, Multiple Shoot Induction and ex vitro Establishment of Cymbidium devonianum Paxt.
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How to cite this article:

Potshangbam Nongdam and Nirmala Chongtham, 2011. In vitro Rapid Propagation of Cymbidium aloifolium (L.) Sw.:A Medicinally Important Orchid via Seed Culture. Journal of Biological Sciences, 11: 254-260.

DOI: 10.3923/jbs.2011.254.260






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