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Journal of Biological Sciences

Year: 2011 | Volume: 11 | Issue: 3 | Page No.: 254-260
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Authors

Potshangbam Nongdam

Nirmala Chongtham

Keywords

Research Article

In vitro Rapid Propagation of Cymbidium aloifolium (L.) Sw.:A Medicinally Important Orchid via Seed Culture

Potshangbam Nongdam and Nirmala Chongtham
Cymbidium aloifolium (L.) Sw., an epiphytic orchid is one of the rare orchids of North-Eastern India having high medicinal and ornamental values. The present study was conducted in an attempt to preserve this important orchid by establishing an efficient in vitro regeneration protocol using seed culture. Two culture media viz., Mitra (M) and Murashige and Skoog (MS) supplemented with different plant growth regulators like Indole-3-butyric acid (IBA), 6-Benzyl Amino Purine (BAP) and 1-Nathyl Acetic Acid (NAA) singly or in combination were used for the present study. In 0.5 mg L-1 BAP supplemented media, seed germination percentage was very low and seedling formation was not observed. A similar response was observed when a combination of both IBA and BAP at 0.5 mg L-1 was used. Protocorm development was delayed and insignificant protocorms turned brown in color with the lost of chlorophyll and finally died even after repeated subculturing. Presence of Activated Charcoal (AC) significantly improved seed germination, protocorm formation and shoot multiplication. Shooting was best observed in MS medium supplemented with 1 mg L-1 BAP and AC. Auxins like NAA and IBA proved to be beneficial for rapid root initiation. However, more superior rooting was observed when M medium was enriched with 0.5 mg L-1 of NAA. Healthy seedlings with well developed roots were obtained which were then hardened and transplanted to community pots for further acclimatization to the nursery conditions. The regenerated plants can subsequently be used for commercial and conservation purposes.
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