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Journal of Biological Sciences
  Year: 2010 | Volume: 10 | Issue: 8 | Page No.: 730-738
DOI: 10.3923/jbs.2010.730.738
 
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Effect of Donor Cells Concentration on Colonization of Human Spermatogonial Stem Cells in Recipient Mouse Testes
Tooba Mirzapour, Mansoureh Movahedin, Tengku Azmi Bin Tengku Ibrahim, Abd Wahid Haron, Zohreh Makoolati and Mohamadreza Nowroozi

Abstract:
Exogenesis (cross-species) germ cell transplantation provides an opportunity to investigate fundamental aspects of spermatogenesis. In this study, testis biopsies of patients with maturation arrest of spermatogenesis during a one year ago were first minced mechanically into small pieces and then Spermatogonial Stem Cells (SSCs) and Sertoli cells isolated by the two- step enzymatic digestion, were plated and grown on DSA-Lectin coated dishes in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal calf serum. Transplantation of human spermatogonial cells into mouse recipient testis was performed on day 7 (before colony formation) and 2 weeks after culturing (colony formation). The effects of different concentrations of spermatogonial cell on quantity of transplantation and percent of colonized seminiferous tubules were assayed during 8 weeks after transplantation. The result showed that SSCs can be observed on the basement membrane of the seminiferous tubules in place of spermatogonial stem cells and proliferation occurs about 4 weeks after transplantation. The difference in donor cells concentration had more effect on colonization of mouse recipient testis (p<0.05). It will be an alternative approach for the repopulation of infertile seminiferous tubules and preservation of fertility, in the future.
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How to cite this article:

Tooba Mirzapour, Mansoureh Movahedin, Tengku Azmi Bin Tengku Ibrahim, Abd Wahid Haron, Zohreh Makoolati and Mohamadreza Nowroozi, 2010. Effect of Donor Cells Concentration on Colonization of Human Spermatogonial Stem Cells in Recipient Mouse Testes. Journal of Biological Sciences, 10: 730-738.

DOI: 10.3923/jbs.2010.730.738

URL: https://scialert.net/abstract/?doi=jbs.2010.730.738

 
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