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Journal of Biological Sciences

Year: 2010 | Volume: 10 | Issue: 3 | Page No.: 261-266
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Authors

M.H. Mirmomeni

S. Sajjadi Majd

S. Sisakhtnezhad

F. Doranegard

Keywords

Research Article

Comparison of the Three Methods for DNA Extraction from Paraffin-Embedded Tissues

M.H. Mirmomeni, S. Sajjadi Majd, S. Sisakhtnezhad and F. Doranegard
The aim of this study was to compare three different techniques for optimizing DNA extraction from Paraffin-Embedded Tissues (PETs) in order to generate DNA suitable for PCR. Paraffin-embedded tissues are common resources for molecular genetic studies but the extraction of high quality nucleic acid from them is problematic. DNA extraction methods used include modified phenol-chloroform protocol, salting out method using ammonium acetate and commercial kit (QIA amp DNA Mini Kit (50)-QIAGEN). DNA was extracted using these methods and qualified using spectrophotometer analysis, electrophoresis and PCR amplification. The tissue specimens used for detection of p53 and Nat2 genes consist of liver tissues with hepatocellular carcinoma (HCC) and stomach tissues which showed stomach adenocarcinoma. DNA was extracted using the aforementioned methods. Subsequently, PCR amplification for p53 and Nat2 genes was performed and 196 and 783 bp fragments were yielded for p53 and Nat2 genes, respectively. Finally, comparison of the electrophoresis pattern in all three methods indicated that no significant differences were detected and all three methods showed equally efficient results.
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