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Journal of Biological Sciences
  Year: 2010 | Volume: 10 | Issue: 3 | Page No.: 242-246
DOI: 10.3923/jbs.2010.242.246
 
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Phytochemical Investigation of Methanolic Extract of the Leaves of Pergularia daemia
K. Karthishwaran, S. Mirunalini, G. Dhamodharan, M. Krishnaveni and V. Arulmozhi

Abstract:
The present study deals with the phytochemical examination of therapeutic importance of Pergularia daemia, an important medicinal plant. Medicinal plants are as source of great economic value in the Indian subcontinent and continue to provide valuable therapeutic agents. This study involves the preliminary phytochemical screening, the separation and identification of compounds were present in crude extract of P. daemia leaves by TLC, HPLC and HPTLC. Further, FTIR analysis of the crude extract has been studied. Qualitative analysis of the methanolic extract prepared from P. daemia leaves revealed the presence of alkaloids, flavonoids, tannins, terpenoids, carbohydrates and proteins. A preliminary survey of the scan of the methanolic extract of P. daemia evidenced the presence of multiple components in the extract. The results obtained after qualitative analysis confirmed by spectral analysis. It shows the presence of two major peaks observed in the HPTLC, HPLC and IR spectrum and exhibited the presence of two principle components in the methanolic extract of the leaves. The results suggested that the phytochemical properties of the leaves for curing various ailments. The plant P. daemia is an important source of various types of compounds with diverse chemical structures as well as pharmacological activities.
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How to cite this article:

K. Karthishwaran, S. Mirunalini, G. Dhamodharan, M. Krishnaveni and V. Arulmozhi, 2010. Phytochemical Investigation of Methanolic Extract of the Leaves of Pergularia daemia. Journal of Biological Sciences, 10: 242-246.

DOI: 10.3923/jbs.2010.242.246

URL: https://scialert.net/abstract/?doi=jbs.2010.242.246

COMMENTS
24 April, 2010
Sanghamitra Nayak:
Sir i have done protein extraction and estimation of Pergularia daemia leaves. But I was getting some result from Pergularia daemia leaves.Can i know the proper result of protein content in Pergularia daemia leaves.
18 May, 2010
Karthishwaran.K:

Thanks for your comments. We have just done a priliminary screening of P.daemia leaves in methanolic extract. protein was not found in the methanolic extract due to the colour inference. At present we have investigated on the ariel parts (flower, leaves)of the plant were we found protein content in methanolic extract. Regards Karthish

19 May, 2010
Sanghamitra Nayak:

Thank u sir responding my comments towards P.daemia Plant.Another thing Atropine is the major Phytochemical present in this plant. Sir do have any knowledge for the spectral analysis of atropine. Or any protocol for the atropine analysis.

31 July, 2012
venkatesh:
sir I want to do my project in p.daemia leaves for antidiabetic activity .so I know availability of this leaves . kindly help me
02 August, 2012
Karthishwaran. K:

Plants are available in south india, especially in South Tamilnadu.

10 August, 2012
venkatesh:

thank you sir for responding my comments

14 March, 2019
Abdul Wadood Chishti:
sir,
I want to investigate the phytoconstituents from such a plant whose constitutional study have never been done before.I am the first one to investigate the constituents.How should i do.kindly share litrature or experimentals so that i can perform my activity.

Thanks
 
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