
	Journal of Biological Sciences
	Year: 2010 \| Volume: 10 \| Issue: 3 \| Page No.: 178-189 DOI: 10.3923/jbs.2010.178.189

Isolation of Aspergillus sulphureus, Penicillium islandicum and Paecilomyces variotii from Agricultural Soil and their Biological Activity Against Pythium spinosum, the Damping-Off Organism of Soybean

Hashem Al-Sheikh and H.M.A. Abdelzaher

Abstract: This research was undertaken to study the biological control of Pythium spinosum var. spinosum, the damping-off organism of soybean using three selected soil fungi of Aspergillus sulphureus, Penicillium islandicum and Paecilomyces variotii. Pythium spinosum var. spinosum was isolated from rhizosphere soil and rhizoplane of healthy and infected soybean roots cultivated in an agricultural field located in Shahean district, Minia city, Egypt in June of 2003 and 2009 using NARM (Nystatin Ampicillin Rifampcin Miconazole) selective medium. Rhizosphere and rhizoplane mycoflora isolated from the same sites were tested for their antagonism against Pythium spinosum in agar plates. Among the isolated fungi, Aspergillus sulphureus, Penicillium islandicum and Paecilomyces variotii were proved to be potent biological agents in the plate assay. Their field effectiveness was evaluated in either autoclaved or nonsterilized soil. Coating soybean seeds and roots with spores and mycelia of these three antagonists gave germinating seeds and seedlings a very good protection from root-rot, pre- and post-emergence damping-off caused by P. spinosum. Applying these biocontrol agents to autoclaved and nonsterilized soil infested with P. spinosum provided an excellent way of protection.

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How to cite this article:

Hashem Al-Sheikh and H.M.A. Abdelzaher, 2010. Isolation of Aspergillus sulphureus, Penicillium islandicum and Paecilomyces variotii from Agricultural Soil and their Biological Activity Against Pythium spinosum, the Damping-Off Organism of Soybean. Journal of Biological Sciences, 10: 178-189.

DOI: 10.3923/jbs.2010.178.189

URL: https://scialert.net/abstract/?doi=jbs.2010.178.189

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