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Journal of Biological Sciences
  Year: 2010 | Volume: 10 | Issue: 2 | Page No.: 145-150
DOI: 10.3923/jbs.2010.145.150
Molecular Characterization of Hemagglutinin and Neuraminidase Genes of H9N2 Avian Influenza Viruses Isolated From Commercial Broiler Chicken in Iran
M. Soltanialvar, H. Shoushtari, M. Bozorgmehrifard, S. Charkhkar and F. Eshratabadi

Abstract:
A total of 512 tissue samples collected from 30 farms located in various states of Iran during 2008-2009 as part of a program to monitor AIV infection in Iran poultry population. Avian influenza virus was isolated from poultry farms with history of respiratory illness and increased mortality. Nucleotide sequence analysis of five representative isolates confirmed that all isolates possessed one type of amino acid motif (R-S-S-R/GL) at cleavage site of HA, Deduced amino acid sequences showed the presence of L226 (234 in H9 numbering) in all five Iranian isolates which indicates a preference to binding of α (2-6) sialic acid receptors. The viruses' surface glycoproteins genes were >90% similar to those of A/Quail/Hong Kong/G1/97 (H9N2) lineage. The neuraminidase stalks regions in these viruses had no deletion as compared to that A/Dk/HK/ Y280/97 lineage (Ck/Bei-like viruses) and the 2 human isolates A/HK/1073/99, A/HK/1074/99. The hemadsorbing site of neuraminidase had up to 3 amino acid substitutions and is different from those of earlier Iranian viruses. Phylogenetic analysis HA and NA genes showed that they share a common ancestor Qa/HK/G1/97 isolate which had contributed internal genes of H5N1 virus.
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How to cite this article:

M. Soltanialvar, H. Shoushtari, M. Bozorgmehrifard, S. Charkhkar and F. Eshratabadi, 2010. Molecular Characterization of Hemagglutinin and Neuraminidase Genes of H9N2 Avian Influenza Viruses Isolated From Commercial Broiler Chicken in Iran. Journal of Biological Sciences, 10: 145-150.

DOI: 10.3923/jbs.2010.145.150

URL: https://scialert.net/abstract/?doi=jbs.2010.145.150

 
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