Antioxidation and Hypomethylation Effects on Genotoxicity and Programmed Cell Death Induced in Mice Somatic Cells by Arsenic Trioxide


	Journal of Biological Sciences
	Year: 2009 \| Volume: 9 \| Issue: 7 \| Page No.: 721-729 DOI: 10.3923/jbs.2009.721.729

Antioxidation and Hypomethylation Effects on Genotoxicity and Programmed Cell Death Induced in Mice Somatic Cells by Arsenic Trioxide

Saad Alkahtani

Abstract: The present study aims to evaluate the effect of antioxidation and hypomethylation on the genotoxicity and apoptosis induced in mice by arsenic trioxide (As₂O₃) in normal adult male SWR/J mouse. Animals were treated with intraperitoneally (ip) injected with (2.65, 5.35 or 10.70 mg kg^-1 b.wt. of As₂O₃ which represent 0.25, 0.50 or 1 of LD₅₀, respectively) and killed 24 h later. Another groups were treated with 30 mg kg^-1 b.wt. of antioxidant and hypermethylizing agent butylated hydroxy toluene (BHT) 1 h prior to As₂O₃ administration. Another different groups were treated with three doses of 5-azacitidine (5-AzaC) 5 mg kg ^-1 b.wt. As₂O₃ administered after 6 days of the last dose. The three single doses of As₂O₃ significantly (p<0.05) increased the rate of total structural chromosomal aberrations (CAs) compared with the negative control. No significant effect was observed in the combined treatment with BHT or 5-AzaC compared with single treatments. The histopathological analyses of mice liver cells showed significantly (p<0.05) increased in apoptosis markers in all three single doses of As₂O₃ compared with the negative control and also significantly (p<0.05) increased with combined treatment with BHT at low and high doses compared with single doses. This study showed that administration of As₂O₃ had a negative effects as represented in CAs test, antioxidant as represented in apoptosis markers and 5-AzaC as represented in rate of pulverized chromosomes, centromeric attenuations, number of polyploidy cells.

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How to cite this article:

Saad Alkahtani , 2009. Antioxidation and Hypomethylation Effects on Genotoxicity and Programmed Cell Death Induced in Mice Somatic Cells by Arsenic Trioxide. Journal of Biological Sciences, 9: 721-729.

DOI: 10.3923/jbs.2009.721.729

URL: https://scialert.net/abstract/?doi=jbs.2009.721.729

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