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Journal of Biological Sciences
  Year: 2009 | Volume: 9 | Issue: 5 | Page No.: 488-493
DOI: 10.3923/jbs.2009.488.493
Phytochemical and Cytotoxic Investigations of Pereskia grandifolia Haw. (Cactaceae) Leaves
A.M. Sri Nurestri, K.S. Sim and A.W. Norhanom

Abstract:
Pereskia grandifolia Haw. (Cactaceae) was investigated for its cytotoxic effect against five human carcinoma cell lines, namely nasopharyngeal epidermoid carcinoma cell line (KB), cervical carcinoma cell line (CasKi), colon carcinoma cell line (HCT 116), hormone-dependent breast carcinoma cell line (MCF7), lung carcinoma cell line (A549) and the non-cancer human fibroblast cell line (MRC-5) using in vitro cytotoxicity assay. The crude methanol and its fractionated extracts (hexane, ethyl acetate and water) have no cytotoxic effect against MRC-5 cells. The hexane fraction of Pereskia grandifolia possessed remarkable cytotoxic effect against KB cells with IC50 values of 5.0 μg mL-1 while ethyl acetate fraction showed high cytotoxic effect against KB and MCF7 cells with IC50 values of 16.0 and 20.0 μg mL-1, respectively. β-Sitosterol (1), vitamin E (2), phytone (3), 2,4-ditert-butylphenol (4) and a mixture consisting of 2,4-ditert-butylphenol (4), methyl palmitate (5), methyl oleate (6) and methyl stearate (7), isolated from the active ethyl acetate fraction were also evaluated for their cytotoxic activity. Compound (4) possessed very remarkable cytotoxic activity against KB cells with IC50 value (concentration of test compounds that cause 50% inhibition or cell death) of 0.81 μg mL-1. This is the first report of the cytotoxic activity of Pereskia grandifolia.
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How to cite this article:

A.M. Sri Nurestri, K.S. Sim and A.W. Norhanom, 2009. Phytochemical and Cytotoxic Investigations of Pereskia grandifolia Haw. (Cactaceae) Leaves. Journal of Biological Sciences, 9: 488-493.

DOI: 10.3923/jbs.2009.488.493

URL: https://scialert.net/abstract/?doi=jbs.2009.488.493

 
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