In vitro Effects of Gliclazide and Metformin on the Protein Binding of Caffeine in the Aqueous Media

Research Article

In vitro Effects of Gliclazide and Metformin on the Protein Binding of Caffeine in the Aqueous Media

Mohammad Mohiuddin, A.T.M. Zafrul Azam, Md. Shah Amran and Md. Amjad Hossain

The in vitro study of protein binding of caffeine and its 1:1 mixtures with gliclazide and metformin HCI has been conducted by equilibrium dialysis method performing measurement by direct spectrophotometric method at temperature 37± 0.5°C and at pH 7.4. In this study, number of binding sites and affinity constants of caffeine and its 1:1 mixture with gliclazide and metformin HCI were calculated by scatchard method. Scatchard plots were prepared to reveal the number of binding sites and the affinity for protein binding. It has been found that both gliclazide and metformin HCI cause lowering the affinity and percentage of binding of the drugs in the mixture to bovine serum albumin. Thus, the interaction of caffeine with gliclazide and metformin HCl can increase the free drug concentration of caffeine in blood plasma. This may change the pharmacokinetic and pharmacodynamic properties of the drug.

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Mohammad Mohiuddin, A.T.M. Zafrul Azam, Md. Shah Amran and Md. Amjad Hossain, 2009. In vitro Effects of Gliclazide and Metformin on the Protein Binding of Caffeine in the Aqueous Media. Journal of Biological Sciences, 9: 476-481.

DOI: 10.3923/jbs.2009.476.481

URL: https://scialert.net/abstract/?doi=jbs.2009.476.481

Journal of Biological Sciences

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Authors

Mohammad Mohiuddin

A.T.M. Zafrul Azam

Md. Shah Amran

Md. Amjad Hossain

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Research Article

In vitro Effects of Gliclazide and Metformin on the Protein Binding of Caffeine in the Aqueous Media

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