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Journal of Biological Sciences
  Year: 2009 | Volume: 9 | Issue: 5 | Page No.: 423-430
DOI: 10.3923/jbs.2009.423.430
Determination of the Toxicity Effect of Sorbitan Esters Surfactants Group on Biological Membrane
G. Dehghan Noudeh, P. Khazaeli, S. Mirzaei, F. Sharififar and S. Nasrollahosaiani

Abstract:
The aim of this study is to investigate the effect of one group of surfactants including sorbitan esters (span 20, 40, 60 and 80) on biologic membrane by using human red blood cells as a model. In this study, the hemolytic effect of four surfactants from span category were evaluated. Surfactants solution were prepared in Mclvane’s buffer in specific concentration. Red blood cells were mixed with surfactants solution incubated in three different time and three temperature. The UV absorbances of the samples were determined by UV spectrophotometer. Each test was done in 9 times. Emulsification index, foam producing activity, also determined for each surfactant solution. The results showed that these agents had hemolytic effects. Span 20 caused more hemolysis and membrane demolishing effect than the others. Their hemolysis effects were enhanced by increasing temperature and time of incubation. According to hemolysis results, foam activity test and E24, the interaction between span 80 and biological membrane is the least and it means has least toxic effect on cells. Anyway because these agents are used as excipients in pharmaceutical preparations, their membrane toxic effect should be considered.
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How to cite this article:

G. Dehghan Noudeh, P. Khazaeli, S. Mirzaei, F. Sharififar and S. Nasrollahosaiani, 2009. Determination of the Toxicity Effect of Sorbitan Esters Surfactants Group on Biological Membrane. Journal of Biological Sciences, 9: 423-430.

DOI: 10.3923/jbs.2009.423.430

URL: https://scialert.net/abstract/?doi=jbs.2009.423.430

COMMENTS
20 December, 2012
Miloni:
Dear Sir,
I want to do a similar study but have a few questions to ask. For the emulsification index you have considered and studied the E24 at various concentrations of surfactants. However, you have not mentioned as to in which system you made these concentrations. Could you please highlight on the solvent system used. Or did you simply use water? Also is it important to study the E24 index by varying the concentrations or pH of the emulsion system?? Also how did you decide the varying concentrations of the surfactants for the toxicity study? Was it based simply on CMC? Should the concentration be considered logarithmically from the CMC value?

I would be grateful if you could answer my questions.

Regards,
Miloni
 
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