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Journal of Biological Sciences
  Year: 2009 | Volume: 9 | Issue: 5 | Page No.: 392-401
DOI: 10.3923/jbs.2009.392.401
Effective Inactivation of Edwardsiella tarda for the Development of Vaccine for Fish
Md. Mer Mosharraf Hossain, Kenji Kawai and Syunichirou Oshima

Abstract:
To find out adequate inactivator in substitution for the high concentration of formalin, different organic and inorganic chemicals, heat and chemicals combined with heat were tested for the inactivation of a fish-pathogenic bacterium Edwardsiella tarda strain. The survivality, sustainability of the cell surface antigenicity, bacterial protein and the total cell antigens of E. tarda were determined after treated with those inactivators. A major antigen at 37 kDa was detected by Sodium Dodecyl Sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and Western blot analysis. Formalin (0.1%) with heat (70°Cx10 min) and 0.9% citric acid killed E. tarda resulted moderate killing activity with enough antigen-sustainability. This study suggests that formalin (0.1%) combined with heat (70°Cx10 min) and citric acid (0.9%) killed E. tarda as a new vaccine candidate.
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How to cite this article:

Md. Mer Mosharraf Hossain, Kenji Kawai and Syunichirou Oshima, 2009. Effective Inactivation of Edwardsiella tarda for the Development of Vaccine for Fish. Journal of Biological Sciences, 9: 392-401.

DOI: 10.3923/jbs.2009.392.401

URL: https://scialert.net/abstract/?doi=jbs.2009.392.401

 
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