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Journal of Biological Sciences
  Year: 2009 | Volume: 9 | Issue: 3 | Page No.: 268-271
DOI: 10.3923/jbs.2009.268.271
Phytoplasmas Associated to Diseases of Ornamental Cacti in Mexico
K. Avina-Padilla, F. Parra-Cota, J.C. Ochoa-Sanchez, C. Perales-Segovia and J.P. Martinez-Soriano

Abstract:
This study was aimed to elucidate the putative causal agents. Healthy and diseased Echinopsis sp. and Opuntia sp. plants were collected from several Mexican nurseries. DNA was extracted from proliferating buds or stems and used in Polymerase Chain Reactions (PCR) to detect phytoplasmas. Two universal phytoplasma primer pairs were tested in nested PCR, initially with primer pair P1/tint followed by primer pair R16F2/R16R2 in a sequential test. The amplified DNA fragments were cloned and sequenced. Two different 16S rDNA partial operons were determined. One of present sequences was always associated to yellow mosaics in Echinopsis while the other to witches-broom syndromes in Opuntia. The comparative analysis of the sequences against the GenBank indicated that they were highly but not 100% homologous to phytoplasmas of group 16SrII. This is of major importance since this is the first report of finding representatives of this group affecting cacti in the Americas; all other reports of this kind of phytoplasmas have been detected in mainly in Asia.
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How to cite this article:

K. Avina-Padilla, F. Parra-Cota, J.C. Ochoa-Sanchez, C. Perales-Segovia and J.P. Martinez-Soriano, 2009. Phytoplasmas Associated to Diseases of Ornamental Cacti in Mexico. Journal of Biological Sciences, 9: 268-271.

DOI: 10.3923/jbs.2009.268.271

URL: https://scialert.net/abstract/?doi=jbs.2009.268.271

 
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