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Journal of Biological Sciences

Year: 2009 | Volume: 9 | Issue: 1 | Page No.: 12-20
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Authors

L. Zakaria

N.S. Ali

B. Salleh

M. Zakaria

Keywords

Research Article

Molecular Analysis of Ganoderma species from Different Hosts in Peninsula Malaysia

L. Zakaria, N.S. Ali, B. Salleh and M. Zakaria
Ganoderma isolates from different hosts namely, G. boninense from oil palm (Elaeis guineensis), G. philiplii from rubber (Hevea brasiliensis) and G. australe from forest trees were characterized using RAPD and PCR-RFLP of ITS+5.8S regions. RAPD primers of high G+C content of 80-100% [CRL-1 (5’CCAGCGCCCC), CRL-2 (5`CTGCCGCCGC), CRL-7 (5’GCCCGCCGCC3’), CRL-11 (5`CCACCGCGCC) and CRL-34 (5’GACCGCGCCC)] showed that the banding patterns from the same species generated similar patterns. Like RAPD, restriction analysis of ITS+5.8S regions using six restriction enzymes (Mspl, Bsu151, Hin61, Hindlll, Hinfl and Taql) also showed that restriction patterns from the same species generated similar patterns. From UPGMA cluster analysis of RAPD and PCR-RFLP of ITS+5.8S regions, Ganoderma species from the same host were clustered together. The results from the present study showed that RAPD and PCR-RFLP of ITS+5.8S regions could be used in characterization and taxonomic analysis of Ganoderma species from different hosts. Both techniques could also provide rapid procedure for differentiation of Ganoderma species in Peninsula Malaysia.
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