Ganoderma isolates from different hosts namely,
G. boninense from oil palm (Elaeis guineensis), G. philiplii
from rubber (Hevea brasiliensis) and G. australe from forest
trees were characterized using RAPD and PCR-RFLP of ITS+5.8S regions.
RAPD primers of high G+C content of 80-100% [CRL-1 (5CCAGCGCCCC), CRL-2
(5`CTGCCGCCGC), CRL-7 (5GCCCGCCGCC3), CRL-11 (5`CCACCGCGCC) and CRL-34
(5GACCGCGCCC)] showed that the banding patterns from the same species
generated similar patterns. Like RAPD, restriction analysis of ITS+5.8S
regions using six restriction enzymes (Mspl, Bsu151, Hin61,
Hindlll, Hinfland Taql) also showed that
restriction patterns from the same species generated similar patterns.
From UPGMA cluster analysis of RAPD and PCR-RFLP of ITS+5.8S regions,
Ganoderma species from the same host were clustered together. The
results from the present study showed that RAPD and PCR-RFLP of ITS+5.8S
regions could be used in characterization and taxonomic analysis of Ganoderma
species from different hosts. Both techniques could also provide rapid
procedure for differentiation of Ganoderma species in Peninsula
How to cite this article
L. Zakaria, N.S. Ali, B. Salleh and M. Zakaria, 2009. Molecular Analysis of Ganoderma species from Different Hosts in Peninsula Malaysia. Journal of Biological Sciences, 9: 12-20.