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The Ultra Structural Study of Blastema in Pinna Tissues of Rabbits with Transmission Electron Microscope |
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Nasser Mahdavi Shahri,
Fatemeh Naseri,
Masoumeh Kheirabadi,
Sakineh Babaie,
Fereshteh Sadeghie Shakib and Mahnaz Azarniya |
Abstract:
The aim of this study is to introduce an experimental
model to produce blastema tissue and to prepare samples for distinction
studies with Transmission Electron Microscope (TEM). Blastema tissue is
a group of undifferentiated cells that are able to divide and differentiate
in some parts of the body. New Zealand White male rabbits with body weight
of less than 2.5 kg and age of nearly 6 months were used in this study.
At first with the help of punching techniques 4.5 mL holes were produced
in rabbits` pinnas and then in 4, 5, 6, 7, 11, 21 and 24 days after regeneration
the tissues around the punching holes were biopsied, using a grasp with
more diameter than the one which the holes had been caused by and then
samples were prepared for histological studies with electron and objective
microscopes. Qualitative and quantitative investigations of electron micrographs
demonstrated that in punching site dedifferentiation of blastema cells
was obvious. The number of cells of each
type were counted and they were compared
with each other. The surface of organelles such as cytoplasm, nucleus,
endoplasmic reticulum, golgi sacs, mitochondrions, lysosoms and light
and dark vacuoles were also measured and compared and then the related
data were studied statistically. According to the
results obtained from morphologic (cytological) and quantitative studies
(comparison between different cellular organelles), The development of
Blastema tissue cells regarding the time of study is obvious, so that
the existence of chondroblast cells in chondrogenesis and endothelial
cells in angiogenesis in 11 and 24 days after regeneration can be seen.
So, considering the importance of understanding the blastema cells ultrastructure
in mammals, morphology of their development and the fact that it has not
been reported so far, this study is the first step and can be continued
by other researchers.
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How to cite this article:
Nasser Mahdavi Shahri, Fatemeh Naseri, Masoumeh Kheirabadi, Sakineh Babaie, Fereshteh Sadeghie Shakib and Mahnaz Azarniya, 2008. The Ultra Structural Study of Blastema in Pinna Tissues of Rabbits with Transmission Electron Microscope. Journal of Biological Sciences, 8: 993-1000.
DOI: 10.3923/jbs.2008.993.1000
URL: https://scialert.net/abstract/?doi=jbs.2008.993.1000
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