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| Effect of Nigella sativa Oil on Metabolic Responses to Prolonged Systemic Injury in Rats |
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Mohammed A. Alsaif |
Abstract:
The objective of the present study was to investigate
the metabolic response to prolonged systemic injury in Wistar rats supplemented
with Nigella sativa L. (N. sativa) oil and/or
saturated fat. Forty-eight animals were divided in to three dietary groups;
Rat-Chow (RC), N. sativa oil diet (NSOD) (Combination of 4% N.
sativa oil and 16% butter oil) and saturated fat diet (SFD) (20% butter
oil) and diets were supplemented for six weeks. At end of the dietary
treatments, each group was divided in two groups as control and trauma.
The systemic injury induced with three subcutaneous injections of turpentine
(2 mL kg-1) in the dorso-lumber region by keeping 48 h intervals
between the injections. TNF-α, IL-6, AST, ALT, ACP, ALP, glucose,
triglycerides, cholesterol, albumin and total protein concentrations were
estimated in serum by using commercially available kits. Nucleic acid
(RNA), GSH and MDA levels were estimated in tissues. Mean body and visceral
fat weights were significantly increased in SFD fed animals while it found
remain unchanged in NSOD fed rats. Serum enzymes, interleukins and lipids
levels were significantly elevated in trauma groups, the least increase
was found in NSOD fed rats compared SFD fed injured rats. RNA, total proteins
and GSH levels decreased and MDA levels increased significantly in SFD
fed traumatized rats compared to NSOD fed animals. The results revealed
with histopathological evaluation found protective effect of N. sativa
oil against the trauma-induced metabolic changes. In conclusion, N.
sativa oil is more beneficial for accidental or post-surgical patients.
Further studies are required to find out its effects over chronic HFD
exposure along with chemically-induce pro-oxidation process.
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How to cite this article:
Mohammed A. Alsaif , 2008. Effect of Nigella sativa Oil on Metabolic Responses to Prolonged Systemic Injury in Rats. Journal of Biological Sciences, 8: 974-983.
DOI: 10.3923/jbs.2008.974.983
URL: https://scialert.net/abstract/?doi=jbs.2008.974.983
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