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Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 6 | Page No.: 974-983
DOI: 10.3923/jbs.2008.974.983
Effect of Nigella sativa Oil on Metabolic Responses to Prolonged Systemic Injury in Rats
Mohammed A. Alsaif

The objective of the present study was to investigate the metabolic response to prolonged systemic injury in Wistar rats supplemented with Nigella sativa L. (N. sativa) oil and/or saturated fat. Forty-eight animals were divided in to three dietary groups; Rat-Chow (RC), N. sativa oil diet (NSOD) (Combination of 4% N. sativa oil and 16% butter oil) and saturated fat diet (SFD) (20% butter oil) and diets were supplemented for six weeks. At end of the dietary treatments, each group was divided in two groups as control and trauma. The systemic injury induced with three subcutaneous injections of turpentine (2 mL kg-1) in the dorso-lumber region by keeping 48 h intervals between the injections. TNF-α, IL-6, AST, ALT, ACP, ALP, glucose, triglycerides, cholesterol, albumin and total protein concentrations were estimated in serum by using commercially available kits. Nucleic acid (RNA), GSH and MDA levels were estimated in tissues. Mean body and visceral fat weights were significantly increased in SFD fed animals while it found remain unchanged in NSOD fed rats. Serum enzymes, interleukins and lipids levels were significantly elevated in trauma groups, the least increase was found in NSOD fed rats compared SFD fed injured rats. RNA, total proteins and GSH levels decreased and MDA levels increased significantly in SFD fed traumatized rats compared to NSOD fed animals. The results revealed with histopathological evaluation found protective effect of N. sativa oil against the trauma-induced metabolic changes. In conclusion, N. sativa oil is more beneficial for accidental or post-surgical patients. Further studies are required to find out its effects over chronic HFD exposure along with chemically-induce pro-oxidation process.
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How to cite this article:

Mohammed A. Alsaif , 2008. Effect of Nigella sativa Oil on Metabolic Responses to Prolonged Systemic Injury in Rats. Journal of Biological Sciences, 8: 974-983.

DOI: 10.3923/jbs.2008.974.983








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