Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 4 | Page No.: 826-830
DOI: 10.3923/jbs.2008.826.830
Identification of an Isolate of Pseudomonas aeroginosa Deposited in PTCC as a PHA Producer Strain: Comparison of Three Different Bacterial Genomic DNA Extraction Methods
Hamid Mir Mohammad Sadeghi, Ali Jahanian Najafabadi, Daryoush Abedi and Abbas Jafarian Dehkordi

Abstract:
In this study we used a nucleic acid based method to identify the capability of Polyhydroxy alkanoate (PHA) production of two isolates of Pseudomonas aeroginosa deposited in Persian Type Culture Collection (i.e., Pseudomonas aeroginosa PTCC 1310 and 1740) and present results showed this capability for the PTCC1310 but not for the other isolate. This identified isolate could be used for massive production of the mentioned polymers or its pha locus could be cloned and used for the functional expression of the PHA biopolymers. Here we also compared three bacterial genomic DNA extraction methods with respect to their difficulty, cost and the time of procedure. These methods included simple boiling method, phenol-chloroform method and commercial DNA extraction kit. All three methods gave reasonable amount of DNA that could be used as templates for the detection of pha gene. In conclusion we have identified a Pseudomonas aeroginosa strain present in Iran containing the necessary genes for PHA production and suggest that the simple boiling method can be utilized for further screening of bacterial samples for the presence of these genes.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
How to cite this article:

Hamid Mir Mohammad Sadeghi, Ali Jahanian Najafabadi, Daryoush Abedi and Abbas Jafarian Dehkordi, 2008. Identification of an Isolate of Pseudomonas aeroginosa Deposited in PTCC as a PHA Producer Strain: Comparison of Three Different Bacterial Genomic DNA Extraction Methods. Journal of Biological Sciences, 8: 826-830.

DOI: 10.3923/jbs.2008.826.830

URL: https://scialert.net/abstract/?doi=jbs.2008.826.830

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

Curve Bottom