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Journal of Biological Sciences

Year: 2008 | Volume: 8 | Issue: 4 | Page No.: 760-766
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Authors

A.R. Khosravi

M. Riazipour

H. Shokri

M.L. Mousavi

M. Mahmoudi

Keywords

Research Article

Characterization of the Similarity of Protein Patterns and Virulence of Clinical Candida albicans Isolates

A.R. Khosravi, M. Riazipour, H. Shokri, M.L. Mousavi and M. Mahmoudi
The aim of the study was to evaluate the protein similarity degree among 15 C. albicans isolates with different virulence obtained from healthy and infected human and animals. Yeast cells were grown in YPG medium and collected by centrifugation. After cell wall disruption, the cell wall and cytoplasmic proteins were submitted to PAGE and SDS-PAGE techniques. Similarity degree was determined using the Dice similarity coefficient (SD). Candida albicans isolates showed high similarity together with average 86.2% (78.1<SD<100). Regarding to the virulence and host aspects, the means SD of high virulent and low virulent isolates as well as human and animal isolates were 85.8 and 85.6% in PAGE, respectively. Considering SDS-PAGE, electrophoregrams of C. albicans cytoplasmic proteins of low and high virulent isolates as well as human and animal isolates showed the similarity about 90%. The mean SD of cell wall was 91.9% between low virulent and high virulent isolates and 92.2% between human and animal isolates. Such results suggest a highly protein similarity degree among the most isolates with low and high virulence as well as from human and animal hosts. The whole-cell protein profile obtained by these techniques could provide additional criteria for the serologic and immunologic studies of C. albicans.
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