• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Journal of Biological Sciences
  2. Vol 8 (4), 2008
  3. 730-737
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Journal of Biological Sciences

Year: 2008 | Volume: 8 | Issue: 4 | Page No.: 730-737
DOI: 10.3923/jbs.2008.730.737

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 222

Search


Authors


Nurhaida Kamaruddin

Country: Malaysia

Farah-Diba A. Bakar

Country: Malaysia

Nor M. Mahadi

Country: Malaysia

Abdul M.A. Murad

Country: Malaysia

Keywords


  • Colletotrichum gloeosporioides
  • cAMP dependent protein kinase
  • regulatory subunit
  • gene isolation
  • gene expression
Research Article

Isolation and Characterisation of a Gene Encoding the Colletotrichum gloeosporioides Regulatory Subunit of Protein Kinase A

Nurhaida Kamaruddin, Farah-Diba A. Bakar, Nor M. Mahadi and Abdul M.A. Murad
The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kinase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is highly expressed in conidia and appressoria as compared to germinating conidia and mycelia.
PDF Fulltext XML References Citation

How to cite this article

Nurhaida Kamaruddin, Farah-Diba A. Bakar, Nor M. Mahadi and Abdul M.A. Murad, 2008. Isolation and Characterisation of a Gene Encoding the Colletotrichum gloeosporioides Regulatory Subunit of Protein Kinase A. Journal of Biological Sciences, 8: 730-737.

DOI: 10.3923/jbs.2008.730.737

URL: https://scialert.net/abstract/?doi=jbs.2008.730.737

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved