Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 4 | Page No.: 730-737
DOI: 10.3923/jbs.2008.730.737
Isolation and Characterisation of a Gene Encoding the Colletotrichum gloeosporioides Regulatory Subunit of Protein Kinase A
Nurhaida Kamaruddin, Farah-Diba A. Bakar, Nor M. Mahadi and Abdul M.A. Murad

Abstract:
The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kinase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is highly expressed in conidia and appressoria as compared to germinating conidia and mycelia.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Nurhaida Kamaruddin, Farah-Diba A. Bakar, Nor M. Mahadi and Abdul M.A. Murad, 2008. Isolation and Characterisation of a Gene Encoding the Colletotrichum gloeosporioides Regulatory Subunit of Protein Kinase A. Journal of Biological Sciences, 8: 730-737.

DOI: 10.3923/jbs.2008.730.737

URL: https://scialert.net/abstract/?doi=jbs.2008.730.737

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

       

       

Curve Bottom