Research Article

**Isolation and Characterisation of a Gene Encoding the Colletotrichum gloeosporioides Regulatory Subunit of Protein Kinase A**

Nurhaida Kamaruddin, Farah-Diba A. Bakar, Nor M. Mahadi and Abdul M.A. Murad

The isolation and characterization of a gene encoding the regulatory subunit of cAMP-dependent protein kinase (CgPKAR) of the fungal plant pathogen Colletotrichum gloeosporioides is described in this research. The CgPKAR gene and its corresponding cDNA have been cloned and sequenced. Sequence analysis revealed that CgPKAR contains one intron located at position -475 to -539 from the start codon (ATG). The gene encodes a protein comprising of 391 amino acids and showing 90% identity to Colletotrichum lagenarium protein kinase A regulatory subunit. The deduced CgPKAR contains predicted features of type II regulatory subunits which include a serine in the phosphorylation site, a kinase inhibitor domain and two cAMP-binding domains. Southern blot analyses indicated that CgPKAR is a single-copy gene in C. gloeosporioides. Real time RT-PCR analyses indicated that CgPKAR is highly expressed in conidia and appressoria as compared to germinating conidia and mycelia.

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Nurhaida Kamaruddin, Farah-Diba A. Bakar, Nor M. Mahadi and Abdul M.A. Murad, 2008. Isolation and Characterisation of a Gene Encoding the Colletotrichum gloeosporioides Regulatory Subunit of Protein Kinase A. Journal of Biological Sciences, 8: 730-737.

DOI: 10.3923/jbs.2008.730.737

URL: https://scialert.net/abstract/?doi=jbs.2008.730.737

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Authors

Nurhaida Kamaruddin

Farah-Diba A. Bakar

Nor M. Mahadi

Abdul M.A. Murad

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Research Article

**Isolation and Characterisation of a Gene Encoding the Colletotrichum gloeosporioides Regulatory Subunit of Protein Kinase A**

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