Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 3 | Page No.: 683-686
DOI: 10.3923/jbs.2008.683.686
Production and Purification of Polyclonal Antibody Against Human Kappa Light Chain
J. Abdolalizadeh, J. Majidi and S. Farajnia

Abstract:
The aim of this study was production, purification and HRP conjugation of polyclonal IgG against human kappa light chains. Three 6-month-old New Zealand White rabbits were immunized by human kappa light chain. The IgG fraction was purified by ion-exchange chromatography and labeled with Horse Radish Peroxidase (HRP). Direct ELISA was used to determine the optimum titer of HRP conjugated IgG. The purity of various IgG preparations was about 98%. The optimum dilution of prepared HRP conjugated IgG was 1:16000. The produced antibody has many application in research, clinic and education. This polyclonal antibody can be used for diagnosis and monitoring of free light chain producing diseases.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Detecting Splicing Variants of FOXP2 and its Protein Expression in Chicken Brain
How to cite this article:

J. Abdolalizadeh, J. Majidi and S. Farajnia, 2008. Production and Purification of Polyclonal Antibody Against Human Kappa Light Chain. Journal of Biological Sciences, 8: 683-686.

DOI: 10.3923/jbs.2008.683.686

URL: https://scialert.net/abstract/?doi=jbs.2008.683.686

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

       

       

Curve Bottom