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Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 3 | Page No.: 526-533
DOI: 10.3923/jbs.2008.526.533
The Banana Pulp Polyphenol Oxidase is a Tyrosinase
Fatemh Saeid Nematpour, Kamahldin Haghbeen, Masoumeh Khalili Babaei, Ferdows Rastgar Jazii, Omidreza Nouraeen and Maedeh Baharloui Yancheshmeh

Abstract:
The best extraction result for the polyphenol oxidase (PPO) from the Musa cavendishii pulp was obtained using a phosphate buffer (0.02 M, pH 7) containing PVP (2%), triton X-100 (1%) and ascorbic acid (0.01%). Two active PPO isozymes were obtained applying a procedure involving dialysis, stepwise precipitation, DEAE and Sephadex column chromatography. The most active isozyme cresolase and catecholase activities in the presence of 4-[(4-Methylphenyl)azo]-phenol and caffeic acid showed an 11.67 and 13.3 fold recovery, respectively, after isolation. It exhibited a single band PAGE and had a MW of 58.2 kD and pI of 5.28 with the kinetic parameters of (Km = 18.6 μM and Vmax = 2.8 μM min-1) for caffeic acid and (Km = 0.94 mM and Vmax = 14.8 μM min-1) for dopamine. The presence of dopamine in banana pulp and peel was established and the results were discussed in terms of the identity and physiological role of the banana PPO in a collective and conclusive manner.
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How to cite this article:

Fatemh Saeid Nematpour, Kamahldin Haghbeen, Masoumeh Khalili Babaei, Ferdows Rastgar Jazii, Omidreza Nouraeen and Maedeh Baharloui Yancheshmeh, 2008. The Banana Pulp Polyphenol Oxidase is a Tyrosinase. Journal of Biological Sciences, 8: 526-533.

DOI: 10.3923/jbs.2008.526.533

URL: https://scialert.net/abstract/?doi=jbs.2008.526.533

 
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