Subscribe Now Subscribe Today
Science Alert
FOLLOW US:     Facebook     Twitter
Curve Top
Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 1 | Page No.: 233-235
DOI: 10.3923/jbs.2008.233.235
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Enzymatic Dehalogenation of 2,2-Dichloropropionic Acid by Locally Isolated Methylobacterium sp. HJ1
Ng Hong Jing and Fahrul Huyop

Synthetic halogenated organic compounds are found widely throughout the biosphere due to modern industrial and agricultural processes. Various soils microorganisms are able to utilize halogenated alkanoic acids as a sole carbon source. An active dehalogenase enzyme was demonstrated in the crude extracts of partially purified enzyme from Methylobacterium sp. HJ1. The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with 2,2-dichloropropionic acid with maximum activity in phosphate buffer pH 6.8. The partially purified enzyme was unaffected by Co+, Mg+ or Mn+ ions or glutathione. The enzyme removed chlorides ions present on a number of 2-carbon alkanoic acids if the halogen was on the α but not on the β-position. The putative product of dehalogenation was pyruvate using a standard assay system and at the same time 2,2-dichloropropionic acid depletion was detected in growth medium by High Performance Liquid Chromatography (HPLC).
PDF References Citation Report Citation
  •    Molecular Analysis of Dehalogenase Gene in Genomic DNA of Bacillus megaterium Strain GS1 Isolated from Volcanic Area Gunung Sibayak
  •    Molecular Prediction of Dehalogenase Producing Microorganism using 16S rDNA Analysis of 2,2-dichloropropionate (Dalapon) Degrading Bacterium Isolated from Volcanic Soil
  •    Molecular Identification and Characterization of a Bacterium that has Potential to Degrade Low Concentration of Haloalkanoic Acid
How to cite this article:

Ng Hong Jing and Fahrul Huyop , 2008. Enzymatic Dehalogenation of 2,2-Dichloropropionic Acid by Locally Isolated Methylobacterium sp. HJ1 . Journal of Biological Sciences, 8: 233-235.

DOI: 10.3923/jbs.2008.233.235








Curve Bottom