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Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 7 | Page No.: 1251-1254
DOI: 10.3923/jbs.2008.1251.1254
Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida in Sheep and Goats using Biochemical Tests and Random Amplified Polymorphic DNA (RAPD) Analysis
A.D. Hawari, D.S. Hassawi and M. Sweiss

Abstract:
The aims of this study were to isolate the M. haemolytica and P. multocida from sheep and goats of Jordan and to identify these bacteria by using biochemical tests and molecular technique. One hundred and ninety six samples were collected from nasal and throat of sheep and goats of two regions (Middle and Northern) in Jordan. The results of the biochemical tests and API 20 E kits identified 50 isolates of Pasteurella multocida and 5 isolates of Mannheimia haemolytica. The identification of these isolates was confirmed by polymerase chain reaction (PCR) technique using PMOut primers for P. multocida and PHSSA primers for M. haemolytica and by random amplified polymorphic DNA (RAPD) using the random primer (OPA-11). Fourteen RAPD profiles were found in P. multocida and 2 profiles were found in M. haemolyitca. The results of this study indicated that identification of P. multocida and M. haemolytica by RAPD analysis was in accordance with those of biochemical tests and using of more than one RAPD primers could improve the identification of the isolates.
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How to cite this article:

A.D. Hawari, D.S. Hassawi and M. Sweiss, 2008. Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida in Sheep and Goats using Biochemical Tests and Random Amplified Polymorphic DNA (RAPD) Analysis. Journal of Biological Sciences, 8: 1251-1254.

DOI: 10.3923/jbs.2008.1251.1254

URL: https://scialert.net/abstract/?doi=jbs.2008.1251.1254

 
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