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Journal of Biological Sciences
  Year: 2008 | Volume: 8 | Issue: 7 | Page No.: 1193-1198
DOI: 10.3923/jbs.2008.1193.1198
Molecular Cloning and Characterization of a cDNA Encoding L10 Ribosomal Protein from Mucor racemosus PTCC 5305
N. Setayesh, Z. Sepehrizadeh and M. Tabatabaei Yazdi

Abstract:
The present research aims to study the molecular characterization of a new 60S ribosomal protein L10 (RPL10) from Mucor racemosus PTCC5305 and its coding sequence. A full-length complementary DNA (cDNA) encoding 60S ribosomal protein L10 was isolated from Mucor racemosus cDNA library. The cDNA of 766 base pairs contains a 652 base pairs open reading frame and 5`- and 3`- flanking regions including a polyadenylation sequence. The deduced protein of 216 amino acid residues had an isoelectric point (pI) of 10.16 and a calculated molecular mass of about 24.5 kDa. Sequence analysis of RPL10 revealed 78% identity and 89% similarity with fungus Pyrenophora tritici. Also it showed 73% identity and 84% similarity with Homo sapiens. Phylogenetic tree analysis based on the conserved domain of Ribosomal_L16_L10e super family exhibited that Mucor racemosus RPL10 is closely related to that of Candida glabrata.
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How to cite this article:

N. Setayesh, Z. Sepehrizadeh and M. Tabatabaei Yazdi, 2008. Molecular Cloning and Characterization of a cDNA Encoding L10 Ribosomal Protein from Mucor racemosus PTCC 5305. Journal of Biological Sciences, 8: 1193-1198.

DOI: 10.3923/jbs.2008.1193.1198

URL: https://scialert.net/abstract/?doi=jbs.2008.1193.1198

 
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