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Journal of Biological Sciences

Year: 2008 | Volume: 8 | Issue: 6 | Page No.: 1045-1050
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Authors

R. Vasudevan

P. Ismail

J. Stanslas

N. Shamsudin

Keywords

Research Article

Association of G2350A Polymorphism of Angiotensin Converting Enzyme Gene with Essential Hypertension and Type 2 Diabetes Mellitus in Malaysian Subjects

R. Vasudevan, P. Ismail, J. Stanslas and N. Shamsudin
The objective of this study was to determine the association of Angiotensin-Converting Enzyme (ACE) G2350A polymorphism in Essential Hypertension (EHT) and Type 2 Diabetes Mellitus (T2DM) in Malaysian subjects. A total of 265 samples have been recruited for this study. In order to amplify the 122 bp of G2350A variant of ACE gene, genomic DNA was amplified using Hot-start Polymerase Chain Reaction (PCR) and the PCR products was digested with BstuI restriction enzyme to produce 103 and 19 bp when A is at position 2350. The subjects were ranged from 30 to 84 years old with a mean age of 51.84 years. The frequency for GG, GA and AA genotypes of ACE gene was 41.43, 48.57 and 10% in EHT, 46.67, 36.67 and 16.66% in T2DM, 32.31, 53.85 and 13.84% in T2DM with EHT compared to 61.43, 35.71 and 2.86% of control subjects respectively. The frequency for A allele of G2350A polymorphism was 34.29% in EHT, 35.00% in T2DM, 40.77% in T2DM+EHT subjects compared to controls (20.71%). The genotype and allele frequency of ACE G2350A gene polymorphism differed significantly in patients when compared to controls (p<0.05). Allele A of G2350A polymorphism of ACE gene is associated with essential hypertension and Type 2 Diabetes Mellitus in Malaysian subjects.
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