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Journal of Biological Sciences
  Year: 2007 | Volume: 7 | Issue: 5 | Page No.: 781-785
DOI: 10.3923/jbs.2007.781.785
Seed Dormancy Breakage of Recalcitrant Yew Species (Taxus baccata L.) Using Embryo Culture
S.M. Hoseini Nasr, S. Modanloo , H. Jalilvand and A.J. Mofidabadi

Abstract:
Two applied methods of seed dormancy breakage of Taxus baccata from mature seed via embryo culture are described. Plant regeneration were obtained by culturing zygotic embryo on MS medium supplemented with 100 mg LG1 casein hydrolysate, 1 g LG1 Yeast extracts, 100 mg LG1 Ascorbic Acid and 5 g LG1 Activated charcoal with pretreatment of 2 weeks seed leaching in running tap water. Another putative plantlet were obtained with one month seed prechilling following embryo culture to WP mineral medium supplemented with B5 vitamins and 5 g LG1 activated charcoal. Development of bipolar structures with recognizable shoot and root apices was observed in embryo culture. Twenty percent of somatic embryos were regenerated in plantlets.
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How to cite this article:

S.M. Hoseini Nasr, S. Modanloo , H. Jalilvand and A.J. Mofidabadi , 2007. Seed Dormancy Breakage of Recalcitrant Yew Species (Taxus baccata L.) Using Embryo Culture. Journal of Biological Sciences, 7: 781-785.

DOI: 10.3923/jbs.2007.781.785

URL: https://scialert.net/abstract/?doi=jbs.2007.781.785

 
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