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Journal of Biological Sciences

Year: 2007 | Volume: 7 | Issue: 4 | Page No.: 663-667
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Authors

Hassan H. Musa

Jin H. Cheng

Wen B. Bao

Sheng L. Wu

Qi Xu

Guo H. Chen

Keywords

Research Article

Assessment of Genetic Diversity and Distance of Lipoprotein Lipase Between Red Jungle Fowl and Domestic Chicken Breeds

Hassan H. Musa, Jin H. Cheng, Wen B. Bao, Sheng L. Wu, Qi Xu and Guo H. Chen
Polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) was used to analyze lipoprotein lipase gene polymorphism in Red Jungle Fowl (Gallus gallus spadiceus), Rugao, Anka, Wenchang and Slikies chicken populations. The results shows high lipoprotein lipase gene diversity, effective number of allele and Shannon information index in Silikes population. The average total genetic diversity (HT), genetic diversity within population (HS) and coefficient of genetic differentiation (GST) across all loci of lipoprotein lipase gene were 0.577±0.013, 0.535±0.011 and 0.073, respectively. The average gene flow across all loci in five chicken populations was 6.332. in addition, the highest lipoprotein lipase genetic distance was 0.220 between Rugao and Anka populations, while the lower was 0.013 between Wenchang and Silikes populations The constructed lipoprotein lipase phylogeny tree shows that Anka and Red jungle fowl were genetically related than the other breeds with 74.4% bootstrapping percentages and then both are related by 74.4% bootstrapping percentages with Wenchang followed by Rugao and Silikes breeds.
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