The Ultrastructural and Stereological Study of Aqueous Extracts of Zataria multiflora Boiss and Elaeagnus angustifolia on the Mouse Fetus Stomach


	Journal of Biological Sciences
	Year: 2007 \| Volume: 7 \| Issue: 4 \| Page No.: 648-652 DOI: 10.3923/jbs.2007.648.652

The Ultrastructural and Stereological Study of Aqueous Extracts of Zataria multiflora Boiss and Elaeagnus angustifolia on the Mouse Fetus Stomach

Monsefi Malihezaman, Mokhberi Mahbobe and Shams Lahijani Maryam

Abstract: Zataria multiflora Boiss (ZmB) and Elaeagnus angustifolia (Ea) are used in traditional folk for stomach tonic. Many pregnant women take these plants to treat the digestive problem that is natural during pregnancy. There were no any reported about the effects of these plants in adult and fetus stomach. In order to investigated the probably effects of theses extracts on the fetus digestive system, 28 pregnant BALB/c mice were divided into control, Sham and experimental groups that treated with 200 mg kg•¹ aqueous extract of ZmB and 640 mg kg•¹ aqueous extract of Ea from 6th to 15th days of pregnancy. The sham group was gavaged with equal amount of distilled water while the control group did not have any treatment. Pregnant mice were sacrificed in 16th day of pregnancy and the fetuses were extracted and their Crown-Rump lengths and weights were measured. Seven fetuses from different mothers were selected and transverse serial sections of their stomach were prepared. The volumes of different layers of stomach were estimated by Cavalieri method. The epithelial and muscular cells of stomach were studied under transmission electron microscopy. There were no significant differences on the volume of stomach after treatment by ZmB but Ea increased the volume of different layers of stomach. No significant differences revealed for weight and CRL of the fetuses. The number of mitochondria of epithelial cells and RER in smooth muscle cells were increased in experimental groups. We concluded that these plants are nonpathogenic and safe and reinforcer for fetuses stomach.

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How to cite this article:

Monsefi Malihezaman, Mokhberi Mahbobe and Shams Lahijani Maryam , 2007. The Ultrastructural and Stereological Study of Aqueous Extracts of Zataria multiflora Boiss and Elaeagnus angustifolia on the Mouse Fetus Stomach. Journal of Biological Sciences, 7: 648-652.

DOI: 10.3923/jbs.2007.648.652

URL: https://scialert.net/abstract/?doi=jbs.2007.648.652

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