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Journal of Biological Sciences

Year: 2007 | Volume: 7 | Issue: 4 | Page No.: 614-619
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Authors

Dewoowoogen Porras Baclayon

Toshiyuki Matsui

Haruo Suzuki

Yusuke Kosugi

Keywords

Research Article

Changes in Activity and Gene Expression of Phenylalanine Ammonia-lyase in Broccoli Branchlets During Early Postharvest Senescence

Dewoowoogen Porras Baclayon, Toshiyuki Matsui, Haruo Suzuki and Yusuke Kosugi
Tissue toughness or textural changes which are closely associated with lignifications in vegetables often provide problem to consumer acceptability particularly those which are consumed raw or half-cooked. To obtain fundamental knowledge underlying this change, enzymatic and molecular analyses of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), enzyme catalyzing the conversion of L-phenylalanine to trans-cinnamic acid in the first step of phenylpropanoid pathway were conducted in the edible branchlets of broccoli during early postharvest senescence. While toughness of the tissue or breaking force as estimated by resistance to pressure on shearing continuously increased, PAL activity decreased after few hours from harvest. The expression of BoPAL was at maximum level on the 12 h from harvest in the upper branchlets. In the lower branchlets, the expression was initially high but declined 6 h from harvest and slightly increased until 24 h of storage. The results imply that, in addition to PAL, other lignin-specific enzymes and/or tissue structural changes may have more significant roles in tissue toughening of broccoli branchlets.
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