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Journal of Biological Sciences

Year: 2007 | Volume: 7 | Issue: 4 | Page No.: 585-591
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Authors

Ikram- ul-Haq

Muhammad Umar Dahot

Keywords

Research Article

Bio-Chemical Contents and Micro-Propagation Efficiency in Banana

Ikram- ul-Haq and Muhammad Umar Dahot
The rate of banana micro-propagation depends on the balanced bio-chemical contents within the cultured explants, which are maintained by specific hormone/s in the medium. A normal mode of organogenesis and than shoot induction, are the hormonal derived steps for in vitro banana micro-propagation. To know about what a situation exist, under specific hormonal conditions, an experiment was designed. The meristematic stem tips were cultured for organogenesis on a number of media supplemented with NAA, BA and IAA separately and/ in a combination. After 2 weeks, the organogenised explants were sub-cultured on shoot induction medium, maximum plantlets (6.20±2.50) were observed on the medium supplemented with BA. Such efficient plant regeneration was occurred, when organogenesis was carried out on the medium supplemented with BA and IAA. Among these explants maximum Na+, Cl•, while decrease in Ca2+, K+, reducing sugar, total sugars and total protein contents were observed. All of these phenomena were reversed in the presence of BA only (shooting culture). These bio-chemical contents in the cultured tissue are specified due to the presence of s balanced form of specific hormone/s in the medium. A specific combination of hormones may be involved to direct organogenesis or shoot induction in the cultured explants.
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