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Journal of Biological Sciences
  Year: 2007 | Volume: 7 | Issue: 1 | Page No.: 57-60
DOI: 10.3923/jbs.2007.57.60
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Nuclear Proteins Associated with Hyphen Growth in Candida albicans

Alsheyab Fawzi

Candida albicans is an opportunistic fungus and the most prevalent among human pathogenic yeasts. The Candida spp. are dimorphic fungi with mycelium (M) and budding yeast (B) growth phases. Dimorphism of C. albicans is believed to be a critical component of pathogenesis, to ensure whether the yeast form or the hyphal form is primary responsible for pathogenicity. Growth-form-specific transcripts of C. albicans were characterized using ddRT-PCR to ascertain their fundamental differentiation process. The isolated transcript gene (cam3) was identified when it`s being expressed during morphogenesis utilizing North blot technique. Differentially expressed mRNAs from both budding and mycelia cultures were characterized for sequence and time of expression during growth phases. One cDNA was identified for transcripts which is apparently unique to hyphal cells. It appears to encode protein homologous to known nuclear proteins. The cDNA (cam3) encodes a polypeptide which shows intriguing similarities to two proteins involved in gene and cell cycle regulation. Portions of the gene align with a protein which interacts with Sin3, a general transcriptional regulator in S. cerevisiae. Other portions appear homologous to proteins involved in uracil anabolism.
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How to cite this article:

Alsheyab Fawzi , 2007. Nuclear Proteins Associated with Hyphen Growth in Candida albicans. Journal of Biological Sciences, 7: 57-60.

DOI: 10.3923/jbs.2007.57.60






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