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Journal of Biological Sciences
  Year: 2007 | Volume: 7 | Issue: 2 | Page No.: 231-238
DOI: 10.3923/jbs.2007.231.238
 
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Sequence Analysis of a Comparative Anchored Tagged Sequence (Biglycan) and its Assignment to the X Chromosome of River Buffalo

Eman R. Mahfouz

Abstract:
Comparative Anchored Tagged Sequences (CATS) are considered to be a valuable tool for comparative mapping and the transfer of genomic information from the well developed maps as human and cattle to other species maps. In an attempt to augment the informational content of the river buffalo physical gene map, a primer pair representing one of the CATS, the Biglycan (BGN), was tested for reaction with river buffalo (Bubalus bubalis, 2n = 50) using Polymerase Chain Reaction (PCR) in order to assign the gene to buffalo chromosomes using a panel of 43 somatic cell hybrids. The segregation panel of BGN with markers representing all bovine synteny groups and the X chromosome revealed its cosegregation with U24 and to a lesser degree with U11 and the X chromosome. The sequence analysis of the buffalo PCR product (amplicon) and Blast searches with GenBank published sequences revealed sequence homology with Bos taurus chromosome X genomic BGN (95% identities) and Homo sapiens chromosome X genomic BGN (91% identities). The obtained results allow the assignment of BGN for the first time to river buffalo chromosome X, thus extending the river buffalo physical map and confirming the extensive genetic conservation between buffalo, cattle and human chromosomes especially for the X chromosome.
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How to cite this article:

Eman R. Mahfouz , 2007. Sequence Analysis of a Comparative Anchored Tagged Sequence (Biglycan) and its Assignment to the X Chromosome of River Buffalo. Journal of Biological Sciences, 7: 231-238.

DOI: 10.3923/jbs.2007.231.238

URL: https://scialert.net/abstract/?doi=jbs.2007.231.238

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