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Journal of Biological Sciences

Year: 2007 | Volume: 7 | Issue: 8 | Page No.: 1511-1519
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Authors

C. Guruvayoorappan

Girija Kuttan

Keywords

Research Article

Antiangiogenic Effect of Rutin and its Regulatory Effect on the Production of VEGF, IL-1β and TNF-α in Tumor Associated Macrophages

C. Guruvayoorappan and Girija Kuttan
The antiangiogenic activity of rutin was studied using in vivo as well as in vitro models. In vivo angiogenic activity was studied using B16F-10 melanoma cell-induced capillary formation in C57BL/6 mice. Administration of rutin significantly inhibited (43.35%) the number of tumor directed capillaries induced by injecting B16F-10 melanoma cells on the ventral side of C57BL/6 mice. Rutin at non-toxic concentrations (5-25 μg mL-1) inhibited the vessel sprouting in rat aortic ring assay. Moreover, rutin was able to inhibit the proliferation, migration and capillary-like tube formation of human endothelial cells, the key steps of angiogenesis. Present studies using Tumor Associated Macrophages (TAMs) revealed that rutin could suppress the expression and production of VEGF and IL-1β and stimulate the production of TNF-α. Hence, the observed antiangiogenic activity of rutin is related to the regulation of these cytokines and growth factors by TAMs.
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