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Journal of Biological Sciences
  Year: 2006 | Volume: 6 | Issue: 2 | Page No.: 398-401
DOI: 10.3923/jbs.2006.398.401
 
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The in vitro Antibacterial Activity of Tinosprora crispa Extracts
Z.A. Zakaria, A.M. Mat Jais, E.F.P. Henie, H. Zaiton, M.N. Somchit, M.R. Sulaiman and F.O. Faizal

Abstract:
The present study was carried out to evaluate the potential of aqueous (AETT), ethanol (EETT) and chloroform (CETT) extracts of Tinosprora crispa as antibacterial agent against selected Gram positive (Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Stretococcus pneumoniae and Clostridium diphtheriae) and Gram negative (Shigella flexneri, Salmonella typhi, Klebsiella pneumoniae, Proteus vulgaris and Escherichia coli) bacteria using the in vitro disc diffusion methods. Twenty microliter of the extracts, prepared in the concentrations of 25, 50, 74 and 100% (stock solution) by diluting the stock solution in distilled water (DH2O) or dimethyl sulfoxide (DMSO), were impregnated on sterilized blank discs (6 mm diameter) and tested against the respective bacteria. The AETT, at all concentrations, was effective only against S. pneumoniae and C. diphtheriae but show an activity against E. coli at the concentrations of 50% and above. At all concentrations used, the EETT was effective against S. aureus, S. pneumoniae, C. diphtheriae and S. flexneri while the CETT was effective against S. pneumoniae, C. diphtheriae and S. flexneri. Furthermore, the CETT, at the concentrations of 50% and above, was effective against E. coli. As a conclusion, T. crispa possesses potential antibacterial properties and further studies are being carried out to isolate and identify the responsible compound.
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How to cite this article:

Z.A. Zakaria, A.M. Mat Jais, E.F.P. Henie, H. Zaiton, M.N. Somchit, M.R. Sulaiman and F.O. Faizal, 2006. The in vitro Antibacterial Activity of Tinosprora crispa Extracts. Journal of Biological Sciences, 6: 398-401.

DOI: 10.3923/jbs.2006.398.401

URL: https://scialert.net/abstract/?doi=jbs.2006.398.401

 
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