Research Article

**Evaluation of the Antioxidant Activity of the Secondary Metabolites from Piliostigma reticulatum (DC.) Hochst**

The ethyl acetate extract of Piliostigma reticulatum leaves was investigated for its antioxidant constituents. Isolated secondary metabolites of the leaves extract include: 2-O-(p-hydroxyphenyl)-5-hydroxy-6-C-methyl-7-methoxychromone (piliostigmin) 1, 6-C- methylkaempferol- 3-methyl ether 2, quercetin 3, quercetrin (quercetin-3-O-rhamnose) 4, and Isoquercetrin (quercetin-3-O-glucoside) 5. These compounds were evaluated for their antioxidant potentials. A rapid test for antioxidants using DPPH (1,1-dipheny-2-picryhydrazyl) TLC screening, demonstrated considerable radical scavenging activity for all the compounds. The DPPH spectrophotometric method was employed for quantitative determination of radical scavenging activity. The activity was expressed as EC₅₀ value, which ranges between 4.68±0.03 and 50.76±1.71 for the flavonoids constituent.

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M.A. Aderogba, E.K. Okoh and T.O. Idowu, 2005. Evaluation of the Antioxidant Activity of the Secondary Metabolites from Piliostigma reticulatum (DC.) Hochst. Journal of Biological Sciences, 5: 239-242.

DOI: 10.3923/jbs.2005.239.242

URL: https://scialert.net/abstract/?doi=jbs.2005.239.242

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Authors

M.A. Aderogba

E.K. Okoh

T.O. Idowu

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Research Article

**Evaluation of the Antioxidant Activity of the Secondary Metabolites from Piliostigma reticulatum (DC.) Hochst**

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