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Journal of Biological Sciences

Year: 2004 | Volume: 4 | Issue: 4 | Page No.: 532-536
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Authors

Naoto Yoshida

Tadahumi Naka

Kazuyoshi Ohta

Keywords

Research Article

Mutagenesis of Bacteria by Fibrous or Clay Minerals

Naoto Yoshida, Tadahumi Naka and Kazuyoshi Ohta
In the present cells of Escherichia coli, Agrobacterium radiobacter, or Thiobacillus intermedius were exposed to chrysotile asbestos, kaolinite, or bentonite by culture in medium containing these minerals. Chromosomal DNA was extracted from exposed cells, then amplified by the RAPD method and band patterns were compared. DNA of bacterial cells exposed to these minerals, was amplified, in whereas that in control cells was not, or that amplified in control cells disappeared. Not only asbestos, but also kaolinite and bentonite, which are principal soil components, induce bacterial mutation. Growth was inhibited by 66%, when A. radiobacter was cultured in LB medium containing 20 mg mL-1 of asbestos for 24 h. Growth inhibition by asbestos was reduced by adding 420 U mL-1 of catalase or 500 U mL-1 of superoxide dismutase to the culture medium.
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