Mutagenesis of Bacteria by Fibrous or Clay Minerals


	Journal of Biological Sciences
	Year: 2004 \| Volume: 4 \| Issue: 4 \| Page No.: 532-536 DOI: 10.3923/jbs.2004.532.536

Mutagenesis of Bacteria by Fibrous or Clay Minerals

Naoto Yoshida , Tadahumi Naka and Kazuyoshi Ohta

Abstract: In the present cells of Escherichia coli, Agrobacterium radiobacter, or Thiobacillus intermedius were exposed to chrysotile asbestos, kaolinite, or bentonite by culture in medium containing these minerals. Chromosomal DNA was extracted from exposed cells, then amplified by the RAPD method and band patterns were compared. DNA of bacterial cells exposed to these minerals, was amplified, in whereas that in control cells was not, or that amplified in control cells disappeared. Not only asbestos, but also kaolinite and bentonite, which are principal soil components, induce bacterial mutation. Growth was inhibited by 66%, when A. radiobacter was cultured in LB medium containing 20 mg mL^-1 of asbestos for 24 h. Growth inhibition by asbestos was reduced by adding 420 U mL^-1 of catalase or 500 U mL^-1 of superoxide dismutase to the culture medium.

PDF References Citation Report Citation

How to cite this article:

Naoto Yoshida , Tadahumi Naka and Kazuyoshi Ohta , 2004. Mutagenesis of Bacteria by Fibrous or Clay Minerals. Journal of Biological Sciences, 4: 532-536.

DOI: 10.3923/jbs.2004.532.536

URL: https://scialert.net/abstract/?doi=jbs.2004.532.536

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRICOLA
		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		EMBASE
		Google Scholar
		SCIMAGO

Follow @scialert

Science Alert