Mechanism of Inhibitory Action of Cyclooxygenase-2 Inhibitors in Human Platelets


	Journal of Biological Sciences
	Year: 2004 \| Volume: 4 \| Issue: 4 \| Page No.: 515-520 DOI: 10.3923/jbs.2004.515.520

Mechanism of Inhibitory Action of Cyclooxygenase-2 Inhibitors in Human Platelets

S.A. Saeed , H. Rasheed , T.M. Ali , Y. Qazi , S. Kabraji , L. Paul , R. Khan , F.A. Hussain and S. Ahmad

Abstract: This study was conducted to investigate the effects of nimesulide in platelet aggregation. It shows that nimesulide (1-100 μ M) inhibited platelets aggregation induced by adrenaline, (20-200 μ M). It also inhibed thromboxane A₂ (TXA₂) formation by platelets at low concentration (IC₅₀; 1 μ M). However, much lower concentrations of nimesulide (0.01-0.1 μ M) potentiated the aggregatory response of subthreshold concentrations of adrenaline (0.2-2 μ M). Such an effect was blocked by Ca²⁺-channel blockers, verapamil and diltiazem (IC₅₀: 7 and 46 μ M, respectively), nitric oxide donor, SNAP (IC₅₀: 2 μ M) and cinchonine (10 nM) but not by genistein (up to 10 μ M). These results are indicative of the concentration-dependent dual effects of nimesulide on human platelet aggregation. The synergistic effect of low doses of nimesulide and adrenaline seems to be mediated through inhibition of multiple signaling pathways.

PDF References Citation Report Citation

RELATED ARTICLES:

•	Reduction in Platelet Aggregation (in vitro) by Diallyl Sulphide in Female Participants with Type 2 Diabetes Mellitus

How to cite this article:

S.A. Saeed , H. Rasheed , T.M. Ali , Y. Qazi , S. Kabraji , L. Paul , R. Khan , F.A. Hussain and S. Ahmad , 2004. Mechanism of Inhibitory Action of Cyclooxygenase-2 Inhibitors in Human Platelets. Journal of Biological Sciences, 4: 515-520.

DOI: 10.3923/jbs.2004.515.520

URL: https://scialert.net/abstract/?doi=jbs.2004.515.520

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRICOLA
		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		EMBASE
		Google Scholar
		SCIMAGO

Follow @scialert

Science Alert