Submit Manuscript

Journal of Biological Sciences

Year: 2003 | Volume: 3 | Issue: 8 | Page No.: 751-759
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 90

Authors

F. Begum

M.N. Amin

S. Islam

M.A.K. Azad

M.M. Rehman

Keywords

Research Article

In vitro Plant Regeneration from Cotyledon-derived Callus  of Three Varieties Pummelo (Citrus grandis [L.] Osb.)

F. Begum, M.N. Amin, S. Islam, M.A.K. Azad and M.M. Rehman
Cotyledon explants from in vitro grown seedlings were cultured on half-strength of MS medium with different growth regulators for in vitro indirect regeneration of shoots. Optimum callus formation, when cotyledon explants were cultured on MS containing 1.0 mg l-1 BAP with 5.0 mg l-1 NAA. After that the calli were used for shoot regeneration by transferring them half-strength MS medium supplemented with only cytokinin. Maximum percentage of shoot regeneration was obtained on half strength MS medium in the presence of 1.0 mg l-1 BAP from callus in three varieties [Var.-1 (pink colour), Var.-2 (white colour) and Var.-3 (red colour)] of pummelo. For rooting, shoot cuttings were cultured on half strength MS salts with 0.1-1.0 mg l-1 NAA, IBA or IAA. The best and healthy rooting was observed on 0.1 mg l-1 NAA. The plantlets of three varieties were successfully established on soil. About 95% of plantlets survived under ex vitro condition.
PDF References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *