In vitro Rapid Regeneration of Plantlets from Leaf Explant of Native-olive (Elaeocarpus robustus Roxb.)

Research Article

In vitro Rapid Regeneration of Plantlets from Leaf Explant of Native-olive (Elaeocarpus robustus Roxb.)

M.M. Rahman, M.N. Amin, M.A.K. Azad, F. Begum and M.Z. Karim

Leaf segments from field grown mature plants of Elaeocarpus robustus Roxb. were used to initiate cultures for inducing callus and subsequent differentiation of shoot buds. Callus production was obtained within seven weeks and adventitious shoot formation was observed after five weeks of incubation on _½ MS medium supplemented with 0.5 mg l^-1 BA + 0.1 mg l^-1 NAA + 15% CW. Complete plantlets were obtained upon transfer of shoot cuttings on _½ MS with 0.1-2.0 mg l^-1 IBA, NAA, IAA or without any auxin. The best and healthy rooting was observed on _½ MS medium supplemented 0.2 mg l^-1 IBA. In vitro regenerated plantlets were transferred to potting soil and successfully established under natural condition with about 60% survival.

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M.M. Rahman, M.N. Amin, M.A.K. Azad, F. Begum and M.Z. Karim, 2003. In vitro Rapid Regeneration of Plantlets from Leaf Explant of Native-olive (Elaeocarpus robustus Roxb.). Journal of Biological Sciences, 3: 718-725.

DOI: 10.3923/jbs.2003.718.725

URL: https://scialert.net/abstract/?doi=jbs.2003.718.725

Journal of Biological Sciences

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Authors

M.M. Rahman

M.N. Amin

M.A.K. Azad

F. Begum

M.Z. Karim

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Research Article

In vitro Rapid Regeneration of Plantlets from Leaf Explant of Native-olive (Elaeocarpus robustus Roxb.)

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