Plant Regeneration from Nodal Segments Derived Callus in Hemidesmus indicus (L.) R. Br (Anantamul) an Endangered Medicinal Plant in Bangladesh


	Journal of Biological Sciences
	Year: 2003 \| Volume: 3 \| Issue: 12 \| Page No.: 1158-1163 DOI: 10.3923/jbs.2003.1158.1163

Plant Regeneration from Nodal Segments Derived Callus in Hemidesmus indicus (L.) R. Br (Anantamul) an Endangered Medicinal Plant in Bangladesh

N.A. Siddique , M.A. Bari , N. Khatun , M. Rahman , M.H. Rahman and S. Huda

Abstract: Callus cultures were initiated from axillary shoots on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-D, NAA, and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg l G ¹ NAA and 2.0 mg l G ¹ Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (86.00%) from axillary shoot derived callus on MS medium containing 1.0 mg l G ¹ NAA and 2.5 mg l G ¹ Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.

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How to cite this article:

N.A. Siddique , M.A. Bari , N. Khatun , M. Rahman , M.H. Rahman and S. Huda , 2003. Plant Regeneration from Nodal Segments Derived Callus in Hemidesmus indicus (L.) R. Br (Anantamul) an Endangered Medicinal Plant in Bangladesh. Journal of Biological Sciences, 3: 1158-1163.

DOI: 10.3923/jbs.2003.1158.1163

URL: https://scialert.net/abstract/?doi=jbs.2003.1158.1163

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