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Journal of Biological Sciences
  Year: 2003 | Volume: 3 | Issue: 12 | Page No.: 1101-1106
DOI: 10.3923/jbs.2003.1101.1106
 
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Callus Induction and Regeneration from Nodal Segment of Potato Cultivar Diamant

N. Khatun , M.A. Bari , R. Islam , S. Huda , N.A.Siddique , M.H. Rahman and M.U. Mollah

Abstract:
The nodal segments of Diamant cultivar of potato from in vitro grown plantlets were cultured for callus induction and regeneration on MS semisolid medium supplemented with different concentrations of 2,4-D, NAA, BAP alone and NAA with BAP. Highest 90.00% of callus formation was observed in MS+2.5 mgl G 12,4-D. The second highest 83.33% callus induction was recorded in MS+5.0 mgl G 1 BAP. Maximum percentages (70.00%) of calli-induced shoots were observed in MS medium fortified with 5.0 mgl G 1 BAP+0.1 mgl G 1IBA. The regenerated shoots were rooted on MS and 2 MS medium containing different concentrations of IBA and maximum rooting response was achieved in 2 MS +1.0 mgl G 1IBA. Regenerated plants were successfully established in soil after acclimatization.
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How to cite this article:

N. Khatun , M.A. Bari , R. Islam , S. Huda , N.A.Siddique , M.H. Rahman and M.U. Mollah , 2003. Callus Induction and Regeneration from Nodal Segment of Potato Cultivar Diamant. Journal of Biological Sciences, 3: 1101-1106.

DOI: 10.3923/jbs.2003.1101.1106

URL: https://scialert.net/abstract/?doi=jbs.2003.1101.1106

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