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Journal of Biological Sciences
  Year: 2001 | Volume: 1 | Issue: 10 | Page No.: 900-901
DOI: 10.3923/jbs.2001.900.901
The Direct Regeneration of Maize Haploids Through Anther Culture
L. Hassan , S.D. Ahmad and A. Okumus

The genetics of plants regenerated from anther-derived callus was investigated using single crosses & inbreds of maize. Anther culture in maize (Zea mays L.) usually follows an indirect procedure involving callus induction and differentiation on at least two different media. Alternatively, development of a direct procedure by which plantlets can be regenerated directly on one medium seems desirable, if acceptable frequency of plant regeneration could be obtained and an effective procedure of chromosome doubling of regenerated plantlets is available. Yupie (YP) and N6 were used as basal media and 10 growth regulators (2,4-D, kinetin, NAA, IAA, IBA, dicamba, pCPA, 6-BA, 2ip and hypoxanthine) were tested to evaluate the feasibility of direct regeneration of haploid plantlets of eight single cross hybrids and four inbred lines. The results indicated that the direct generation of plantlets is feasible through anther culture, though it is genotype and medium dependent. Ten plantlets were regenerated directly from single cross hybrid M60 X M96. The more effective medium was N6 basal medium plus 2,4-D (2.0 mg l-1) and kinetin (1.5 mg l-1). Regeneration frequency (percent of anthers producing plantlets) ranged from 1 to 4%.
PDF References Citation Report Citation
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How to cite this article:

L. Hassan , S.D. Ahmad and A. Okumus , 2001. The Direct Regeneration of Maize Haploids Through Anther Culture. Journal of Biological Sciences, 1: 900-901.

DOI: 10.3923/jbs.2001.900.901








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