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Journal of Biological Sciences

Year: 2001 | Volume: 1 | Issue: 8 | Page No.: 788-790
DOI: 10.3923/jbs.2001.788.790

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Authors


M. Umar Dahot


A.B.Ghanghro


M. Yousuf Khan


Keywords


  • Enzyme
  • phospholipase A
  • melia azadirachta
  • seeds
Research Article

Studies on Phospholipase A from Melia azadirachta (Neem) Seeds Extract

M. Umar Dahot, A.B.Ghanghro and M. Yousuf Khan
The crude extract from dried Melia azadirachta seeds was prepared and it was noted that the seed contains sufficient amount of phospholipase A activity. Optimal activity of Melia azadirachta seeds was found at pH 6.0 and at temperature 35°C. The stability of enzyme was noted over the pH range 5-8. More than 30% activity of phospholipase A remained active up to 60 minutes at 60°C. The enzyme activity was markedly increased in the presence of calcium, manganese and zinc but it was decreased by the addition of EDTA.
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How to cite this article

M. Umar Dahot, A.B.Ghanghro and M. Yousuf Khan, 2001. Studies on Phospholipase A from Melia azadirachta (Neem) Seeds Extract. Journal of Biological Sciences, 1: 788-790.

DOI: 10.3923/jbs.2001.788.790

URL: https://scialert.net/abstract/?doi=jbs.2001.788.790

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