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Journal of Biological Sciences

Year: 2001 | Volume: 1 | Issue: 8 | Page No.: 720-722
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Authors

M. Hassan

Y. Fujime

T. Matsui

N. Okuda

H. Suzuki

Keywords

Research Article

Shoot Regeneration from Wasabi (Wasbia japonica Mtsum) Callus

M. Hassan, Y. Fujime, T. Matsui, N. Okuda and H. Suzuki
Adventitious shoot formation was induced on callus of wasabi. Adventitious buds were formed from subculture of callus on modified MS media (containing 0.002 mg/ l TDZ (n-phenyl-N- 1, 2, 3- thidiazol-5-yl-urea or thidiazuron) and 1 mg/ l of NAA (naphhaleneacetic acid) were failed to elongate. Bud forming calli were subcultured in modified MS media containing BA, (6-benzyladenine) or kinetin with or without NAA or NAA alone. Shoot development was observed on the enlarged callus in media containing BA or kinetin with NAA. While media containing BA or kinetin without NAA and media containing only NAA did not result in organ formation. Calli subcultured on hormone free media caused the buds to become black and die out. Shoots formed in the presence of kinetin were either smaller in size or turned brown in colour. Green and plump shoots were formed in media containing 0.5 or 1 mg/ l of BA with 0.5 mg/ l of NAA. Higher doses of BA and NAA caused the shoots to become thin and branched. Calli that produced the best shoots also grew faster than others having a lush green appearance. However, root formation was less frequent in shoot forming calli. Root formation was most frequent when kinetin was used as a source of cytokinin.
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