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Journal of Applied Sciences

Year: 2023 | Volume: 23 | Issue: 3 | Page No.: 143-153
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Authors

Oloyede Omotade

Alabi Okunola

Alabi Okunola

Akinola Mary

Oni Samuel

Oluboyede Tobi

Adeoluwa Yetunde

Odesanmi Elijah

Afolabi Olakunle

Afolabi Olakunle

Adebo Cosmas

Keywords

Background and Objective: Carica papaya and Bridelia ferruginea are traditionally used in the prevention and management of several diseases. However, limited information exists on the potential adverse effects of these plant extracts on germ cells. This study aimed at investigating the reproductive toxicity of the aqueous extracts of C. papaya (leaves and unripe pulp) and B. ferruginea (stem bark) using a murine sperm morphology assay. Also, the serum biochemical assay of the experimental mice was carried out using selected serum enzymes as biomarkers to evaluate the safety or toxicological potentials of these extracts. Materials and Methods: The reproductive toxicity of the aqueous extracts of C. papaya (unripe pulp and leaves) and B. ferruginea (stem bark) in male mice was evaluated using sperm morphology assay and testes histopathology at 200, 100 and 50 mg kg–1 body weight of each extract. Assessment of selected serum enzymes and oxidative stress biomarkers was also carried out with the analysis of selected sex hormones. Doses were orally administered to the mice daily for seven days. Results: Sperm morphology revealed abnormal sperm cells in the exposed mice in all the doses of the extracts. However, the extract of B. ferruginea significantly (p<0.05) induced sperm abnormality in a dose-dependent manner. Furthermore, all the doses of C. papaya leaf extract also induced significant abnormal sperm cells. Testes histopathology showed alterations of the seminiferous tubule of the exposed mice with varying degrees and types of lesions. The hepatic damage markers (aminotransferases) and renal damage (alkaline phosphatase) significantly increased in mice exposed to the extract of B. ferruginea stem bark. Serum alkaline phosphatase activity was increased in the mice exposed to 100 mg kg–1 body weight C. papaya leaf extract. However, a significant decrease was observed in the catalase and superoxide dismutase activities in the treated mice. A significant increase in follicle stimulating hormone and total testosterone and a decrease in luteinizing hormone was also observed in the exposed mice. Conclusion: Therefore, the findings of the present study indicated that the aqueous extracts of C. papaya leaf and B. ferruginea stem bark could induce toxicity in the germ cells of mice.
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How to cite this article

Oloyede Omotade, Alabi Okunola, Akinola Mary, Oni Samuel, Oluboyede Tobi, Adeoluwa Yetunde, Odesanmi Elijah, Afolabi Olakunle and Adebo Cosmas, 2023. Male Reproductive Toxicity and Modulation of Enzyme Activities by the Extracts of Carica papaya and Bridelia ferruginea. Journal of Applied Sciences, 23: 143-153.

DOI: 10.3923/jas.2023.143.153

URL: https://scialert.net/abstract/?doi=jas.2023.143.153

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